TROPHIC EFFECT OF BETA-AMYLOID PRECURSOR PROTEIN ON CEREBRAL CORTICAL-NEURONS IN CULTURE

被引：197

作者：

ARAKI, W

KITAGUCHI, N

TOKUSHIMA, Y

ISHII, K

ARATAKE, H

SHIMOHAMA, S

NAKAMURA, S

KIMURA, J

机构：

[1] ASAHI CHEM IND CO LTD, LIFE SCI RES LABS, BIOSCI LAB, FUJI, SHIZUOKA 416, JAPAN

[2] KYOTO UNIV, INST VIRUS RES, SAKYO KU, KYOTO 606, JAPAN

[3] HIROSHIMA UNIV, SCH MED, DEPT INTERNAL MED 3, HIROSHIMA 734, JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 181卷 / 01期

关键词：

D O I：

10.1016/S0006-291X(05)81412-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We investigated the effect of human β-amyloid precursor protein (APP) on rat primary cerebral cortical neurons cultured in a serum-free medium. Two secretory APP species (APP667 and APP592) with and without the protease inhibitor domain were produced by COS-1 cells transfected with APP cDNAs, which encode the N-terminal portions of APP770 and APP695. Both highly purified APP species, when added to the medium, enhanced neuronal survival and neurite extension in a dose-dependent manner with a maximum effect at approximately 100 nM. These results suggest that secreted forms of APP have trophic activity for cerebral cortical neurons. © 1991 Academic Press, Inc.

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页码：265 / 271

页数：7

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