DETERMINATION OF AMYLOID BETA-PROTEIN PRECURSORS HARBORING ACTIVE FORM OF PROTEINASE-INHIBITOR DOMAINS IN CEREBROSPINAL-FLUID OF ALZHEIMERS-DISEASE PATIENTS BY TRYPSIN-ANTIBODY SANDWICH ELISA

被引：42

作者：

KITAGUCHI, N ^{[1
]}

TOKUSHIMA, Y ^{[1
]}

OISHI, K ^{[1
]}

TAKAHASHI, Y ^{[1
]}

SHIOJIRI, S ^{[1
]}

NAKAMURA, S ^{[1
]}

TANAKA, S ^{[1
]}

KODAIRA, R ^{[1
]}

ITO, H ^{[1
]}

机构：

[1] KYOTO UNIV,FAC MED,DEPT NEUROL,SAKYO KU,KYOTO 606,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 166卷 / 03期

关键词：

D O I：

10.1016/0006-291X(90)91030-V

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

β-Amyloid protein precursors (APP) having proteinase inhibitor domains (APPI) were quantified by a new sandwich enzyme linked immunosorbent assay for detection of active (free) form of proteinase inhibitors by using trypsin in place of the first antibody and by denaturation of APPI-trypsin complex in the microtiterplate. The concentration of APPs having APPI in cerebrospinal fluid of Alzheimer's disease patients was found, by this method, to be significantly elevated compared with those of multi-infarct dementia. © 1990.

引用

页码：1453 / 1459

页数：7

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