GENOMIC MISMATCH SCANNING - A NEW APPROACH TO GENETIC-LINKAGE MAPPING

被引：98

作者：

NELSON, SF

MCCUSKER, JH

SANDER, MA

KEE, Y

MODRICH, P

BROWN, PO

机构：

[1] STANFORD UNIV, MED CTR, DEPT PEDIAT, STANFORD, CA 94305 USA

[2] STANFORD UNIV, MED CTR, DEPT BIOCHEM, STANFORD, CA 94305 USA

[3] STANFORD UNIV, MED CTR, HOWARD HUGHES MED INST, STANFORD, CA 94305 USA

[4] DUKE UNIV, MED CTR, DEPT BIOCHEM, DURHAM, NC 27710 USA

来源：

NATURE GENETICS | 1993年 / 4卷 / 01期

关键词：

D O I：

10.1038/ng0593-11

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Genomic mismatch scanning (GMS) is a new method of genetic linkage analysis that does not require conventional polymorphic markers or gel electrophoresis. GMS is ideally suited to affected-relative-pair mapping. DNA fragments from all regions of identity-by-descent between two relatives are isolated based on their ability to form extensive mismatch-free hybrid molecules. The genomic origin of this selected pool of DNA fragments is then mapped in a single hybridization step. Here we demonstrate the practicality of GMS in a model organism, Saccharomyces cerevisiae. GMS is likely to be applicable to other organisms, including humans, and may be of particular value in mapping complex genetic traits.

引用

页码：11 / 18

页数：8

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