MAPPING THE WHOLE HUMAN GENOME BY FINGERPRINTING YEAST ARTIFICIAL CHROMOSOMES

被引：168

作者：

BELLANNECHANTELOT, C

LACROIX, B

OUGEN, P

BILLAULT, A

BEAUFILS, S

BERTRAND, S

GEORGES, I

GLIBERT, F

GROS, I

LUCOTTE, G

SUSINI, L

CODANI, JJ

GESNOUIN, P

POOK, S

VAYSSEIX, G

LUKUO, J

RIED, T

WARD, D

CHUMAKOV, I

LEPASLIER, D

BARILLOT, E

COHEN, D

机构：

[1] GENETHON, F-91000 EVRY, FRANCE

[2] INST NATL RECH INFORMAT & AUTOMAT, F-78153 LE CHESNAY, FRANCE

[3] YALE UNIV, SCH MED, DEPT GENET, NEW HAVEN, CT 06510 USA

[4] YALE UNIV, SCH MED, DEPT MOLEC BIOPHYS & BIOCHEM, NEW HAVEN, CT 06510 USA

来源：

CELL | 1992年 / 70卷 / 06期

关键词：

D O I：

10.1016/0092-8674(92)90254-A

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Physical mapping of the human genome has until now been envisioned through single chromosome strategies. We demonstrate that by using large insert yeast artificial chromosomes (YACs) a whole genome approach becomes feasible. YACs (22,000) of 810 kb mean size (5 genome equivalents) have been fingerprinted to obtain individual patterns of restriction fragments detected by a LINE-1 (L1) probe. More than 1000 contigs were assembled. Ten randomly chosen contigs were validated by metaphase chromosome fluorescence in situ hybridization, as well as by analyzing the inter-Alu PCR patterns of their constituent YACs. We estimate that 15% to 20% of the human genome, mainly the L1-rich regions, is already covered with contigs larger than 3 Mb.

引用

页码：1059 / 1068

页数：10

共 33 条

[1] CONSTRUCTION AND CHARACTERIZATION OF A YEAST ARTIFICIAL CHROMOSOME LIBRARY CONTAINING 7 HAPLOID HUMAN GENOME EQUIVALENTS [J].