A TEST CASE FOR PHYSICAL MAPPING OF HUMAN GENOME BY REPETITIVE SEQUENCE FINGERPRINTS - CONSTRUCTION OF A PHYSICAL MAP OF A 420 KB YAC SUBCLONED INTO COSMIDS

被引：13

作者：

BELLANNECHANTELOT, C

BARILLOT, E

LACROIX, B

LEPASLIER, D

COHEN, D

机构：

[1] Centre d'Etude du Polymorphisme Humain, F-75010 Paris

来源：

NUCLEIC ACIDS RESEARCH | 1991年 / 19卷 / 03期

关键词：

D O I：

10.1093/nar/19.3.505

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid and safe method of Yeast Artificial Chromosome (YAC) physical mapping by cosmid 'fingerprinting' is presented. YACs are subcloned into cosmids which are prepared without previous separation of cloned DNA from host DNA. Groups of overlapping clones are detected according to their restriction fragments size and intensity after hybridization with total human DNA. To test this approach, a cosmid library was constructed from total DNA of a yeast strain containing a 420 kb YAC. A single contig of 84 clones was obtained with a minimal detectable overlap of 60% i.e. a 9.2 fold representative library. Large scale physical mapping of YACs would take full advantage of the DNA preparation procedure employed in this work and allows to take into account restriction fragment intensities.

引用

页码：505 / 510

页数：6

共 18 条

[1] IMPROVED CONTROL OF PARTIAL DNA RESTRICTION ENZYME DIGEST IN AGAROSE USING LIMITING CONCENTRATIONS OF MG++ [J].