PURIFICATION OF INITIATOR C FROM ESCHERICHIA COLI - A PROTEIN WHICH BINDS MESSENGER RNA AND INITIATOR TRNA TO 30 S RIBOSOME

被引：40

作者：

HERZBERG, M

LELONG, JC

REVEL, M

机构：

[1] Department of Biological Ultrastructure, Department of Biochemistry Weizmann Institute of Science, Rehovoth

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1969年 / 44卷 / 02期

关键词：

D O I：

10.1016/0022-2836(69)90176-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Attachment of 30 s ribosomes to nascent messenger RNA, which can be studied by electron microscopic quantitation of the number of ribosomes bound to bacteriophage T4 DNA transcribed with RNA polymerase, requires the presence of initiator protein C(F2). On the other hand, C also promotes the binding of N-formylmethionyl-tRNA to the 30 s ribosome. Evidence is presented that both mRNA and initiator tRNA binding activities are due to the same protein. © 1969.

引用

页码：297 / &

共 19 条

[1] A NEW METHOD FOR PURIFICATION OF RNA-POLYMERASE [J].

BABINET, C .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1967, 26 (06) :639-&

[2] DEOXYRIBONUCLEIC ACID-DIRECTED SYNTHESIS OF RIBONUCLEIC ACID BY AN ENZYME FROM ESCHERICHIA COLI [J].

CHAMBERLIN, M ;

BERG, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1962, 48 (01) :81-&

[3] INTERACTION OF GUANOSINE 5'-TRIPHOSPHATE WITH A SUPERNATANT FRACTION FROM E COLI AND AMINOACYL-SRNA [J].

GORDON, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1967, 58 (04) :1574-&

[4] STABILITY OF DIFFERENT RIBOSOMAL COMPLEXES WITH INITIATOR TRANSFER RNA AND SYNTHETIC MESSENGER RNA [J].

GRUNBERG.M ;

CLARK, BFC ;

REVEL, M ;

RUDLAND, PS ;

DONDON, J .

JOURNAL OF MOLECULAR BIOLOGY, 1969, 40 (01) :33-&

[5]

HERZBERG M, 1967, CR ACAD SCI D NAT, V264, P1227

[6] TRANSLATION OF GENETIC MESSAGE .7. ROLE OF INITIATION FACTORS IN FORMATION OF CHAIN INITIATION COMPLEX WITH ESCHERICHIA COLI RIBOSOMES [J].

IWASAKI, K ;

SABOL, S ;

WAHBA, AJ ;

OCHOA, S .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1968, 125 (02) :542-&

[7] RIBOSOME FORMATION FROM SUBUNITS - DEPENDENCE ON FORMYLMETHIONYL-TRANSFER RNA IN EXTRACTS FROM E COLI [J].

KONDO, M ;

EGGERSTON, G ;

EISENSTADT, J ;

LENGYEL, P .

NATURE, 1968, 220 (5165) :368-+

[8] INDEPENDENT TRANSLATION OF GENES OF BACTERIOPHAGE F2 RNA [J].

LODISH, HF .

JOURNAL OF MOLECULAR BIOLOGY, 1968, 32 (03) :681-&

[9] INITIATION OF POLYPHENYLALANINE SYNTHESIS BY N-ACETYLPHENYLALANYL/SRNA [J].

LUCASLENARD, J ;

LIPMANN, F .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1967, 57 (04) :1050-+

[10] TRANSLATION OF TRYPTOPHAN MESSENGER RNA OF ESCHERICHIA COLI [J].

MORSE, DE ;

BAKER, RF ;

YANOFSKY, C .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1968, 60 (04) :1428-&

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