TRANSLATION OF GENETIC MESSAGE .7. ROLE OF INITIATION FACTORS IN FORMATION OF CHAIN INITIATION COMPLEX WITH ESCHERICHIA COLI RIBOSOMES

被引：209

作者：

IWASAKI, K

SABOL, S

WAHBA, AJ

OCHOA, S

机构：

[1] Department of Biochemistry, New York University School of Medicine, New York

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1968年 / 125卷 / 02期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0003-9861(68)90612-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Washing of Escherichia coli Q13 ribosomes with 1.0 m ammonium chloride yields, in addition to the known initiation factors F1and F2, a third factor F3. All three factors are required for translation of natural messenger RNA (mRNA), e.g., phage RNA, in the cell-free E. coli system. They function in formation of the polypeptide chain initiation complex. Incubation of purified ribosomes with 32P-labeled Qβ phage RNA and methionine-14C-labeled formylmethionyl-transfer RNA, at low magnesium ion concentrations, followed by zonal centrifugation analysis in sucrose gradients, showed that formation of the initiation complex involves at least two steps: (1) The F3-dependent binding of mRNA to the ribosome, and (2) the F2-dependent binding of formylmethionyl-transfer RNA to the mRNA-ribosome complex to form the initiation complex proper. © 1968.

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页码：542 / &

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共 11 条

[1] GTP-STIMULATED BINDING OF INITIATOR-TRNA TO RIBOSOMES DIRECTED BY F2 BACTERIOPHAGE RNA [J].

ANDERSON, JS ;

DAHLBERG, JE ;

BRETSCHE.MS ;

REVEL, M ;

CLARK, BFC .

NATURE, 1967, 216 (5120) :1072-&

[2] A SIMPLE EFFICIENT LIQUID SCINTILLATOR FOR COUNTING AQUEOUS SOLUTIONS IN A LIQUID SCINTILLATION COUNTER [J].

BRAY, GA .

ANALYTICAL BIOCHEMISTRY, 1960, 1 (4-5) :279-285

[3] SPECIFIC TEMPLATE REQUIREMENTS OF RNA REPLICASES [J].

HARUNA, I ;

SPIEGELM.S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1965, 54 (02) :579-&

[4] TRANSLATION OF GENETIC MESSAGE .6. ROLE OF RIBOSOMAL SUBUNITS IN BINDING OF FORMYLMETHIONYL-TRNA AND ITS REACTION WITH PUROMYCIN [J].

HILLE, MB ;

MILLER, MJ ;

IWASAKI, K ;

WAHBA, AJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1967, 58 (04) :1652-&

[5] PHAGE F2 RNA-DIRECTED BINDING OF FORMYLMETHIONYL-TRNA TO RIBOSOMES AND ROLE OF 30S RIBOSOMAL SUBUNITS IN INITIATION OF PROTEIN SYNTHESIS [J].

NOMURA, M ;

LOWRY, CV .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1967, 58 (03) :946-&

[6]

REVEL M, IN PRESS

[7] TRANSLATION OF GENETIC MESSAGE .V. EFFECT OF MG++ AND FORMYLATION OF METHIONINE IN PROTEIN SYNTHESIS [J].

SALAS, M ;

MILLER, MJ ;

WAHBA, AJ ;

OCHOA, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1967, 57 (06) :1865-&

[8] TRANSLATION OF GENETIC MESSAGE .2. EFFECT OF INITIATION FACTORS ON BINDING OF FORMYL-METHIONYL-TRNA TO RIBOSOMES [J].

SALAS, M ;

HILLE, MB ;

LAST, JA ;

WAHBA, AJ ;

OCHOA, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1967, 57 (02) :387-&

[9] FORMATION AND STABILIZATION OF 30S AND 50S RIBOSOME COUPLES IN ESCHERICHIA COLI [J].

SCHLESSINGER, D ;

MANGIAROTTI, G ;

APIRION, D .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1967, 58 (04) :1782-+

[10] TRANSLATION OF GENETIC MESSAGE - FACTORS INVOLVED IN INITIATION OF PROTEIN SYNTHESIS [J].

STANLEY, WM ;

SALAS, M ;

WAHBA, AJ ;

OCHOA, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1966, 56 (01) :290-&