STABILITY OF DIFFERENT RIBOSOMAL COMPLEXES WITH INITIATOR TRANSFER RNA AND SYNTHETIC MESSENGER RNA

被引：32

作者：

GRUNBERG.M

CLARK, BFC

REVEL, M

RUDLAND, PS

DONDON, J

机构：

[1] Institut de Biologie Physico-Chimique, Paris, rue P. Curie

[2] Medical Research Council Laboratory of Molecular Biology, Cambridge, England

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1969年 / 40卷 / 01期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0022-2836(69)90294-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Stability of the mRNA-initiator tRNA-ribosomal complex has been studied by nitrocellulose filter binding and thermal dissociation techniques. There is no significant difference between the stability of the 70 s ribosomal complex involving fMet-tRNAf§ and its coding triplet and the stability of other synthetic mRNA-aminoacyl-tRNA systems including the ApUpG-Met-tRNAf complex. However, the 30 s subunit complex with fMet-tRNAf and ApUpG is significantly more stable than Met-tRNAf-ApUpG and other aminoacyl-tRNA-mRNA complexes. Further, the 30 s subunit complex with acetylphenylalanyl-tRNA and poly U is unstable. A stable 70 s ribosomal complex with Met-tRNAm is formed with poly (A,U,G) but not with the triplet ApUpG. © 1969.

引用

页码：33 / &

共 29 条

[1] N-FORMYLMETHIONYL-SRNA AS INITIATOR OF PROTEIN SYNTHESIS [J].