Virus induction of human IFN beta gene expression requires the assembly of an enhanceosome

被引：894

作者：

Thanos, D ^{[1
]}

Maniatis, T ^{[1
]}

机构：

[1] HARVARD UNIV,DEPT MOLEC & CELLULAR BIOL,CAMBRIDGE,MA 02138

来源：

CELL | 1995年 / 83卷 / 07期

关键词：

D O I：

10.1016/0092-8674(95)90136-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We present evidence that transcriptional activation of the human interferon-beta (IFN beta) gene requires the assembly of a higher order transcription enhancer complex (enhanceosome). This multicomponent complex includes at least three distinct transcription factors and the high mobility group protein HMG I(Y). Both the in vitro assembly and in vivo transcriptional activity of this complex require a precise helical relationship between individual transcription factor-binding sites. In addition, HMG I(Y), which binds specifically to three sites within the enhancer, promotes cooperative binding of transcription factors in vitro and is required for transcriptional synergy between these factors in vivo. Thus, HMG I(Y) plays an essential role in the assembly and function of the IFN beta gene enhanceosome.

引用

页码：1091 / 1100

页数：10

共 53 条

[11] COOPERATIVITY AND HIERARCHICAL LEVELS OF FUNCTIONAL-ORGANIZATION IN THE SV40 ENHANCER [J].

FROMENTAL, C ;

KANNO, M ;

NOMIYAMA, H ;

CHAMBON, P .

CELL, 1988, 54 (07) :943-953

[12] EVIDENCE FOR A NUCLEAR FACTOR(S), IRF-1, MEDIATING INDUCTION AND SILENCING PROPERTIES TO HUMAN IFN-BETA GENE REGULATORY ELEMENTS [J].

FUJITA, T ;

SAKAKIBARA, J ;

SUDO, Y ;

MIYAMOTO, M ;

KIMURA, Y ;

TANIGUCHI, T .

EMBO JOURNAL, 1988, 7 (11) :3397-3405

[13] VIRAL INDUCTION OF THE HUMAN BETA-INTERFERON PROMOTER - MODULATION OF TRANSCRIPTION BY NF-KAPPA-B/REL PROTEINS AND INTERFERON REGULATORY FACTORS [J].

GAROUFALIS, E ;

KWAN, I ;

LIN, RT ;

MUSTAFA, A ;

PEPIN, N ;

ROULSTON, A ;

LACOSTE, J ;

HISCOTT, J .

JOURNAL OF VIROLOGY, 1994, 68 (08) :4707-4715

[14] THE HMG DOMAIN OF LYMPHOID ENHANCER FACTOR-I BENDS DNA AND FACILITATES ASSEMBLY OF FUNCTIONAL NUCLEOPROTEIN STRUCTURES [J].

GIESE, K ;

COX, J ;

GROSSCHEDL, R .

CELL, 1992, 69 (01) :185-195

[15] LEF-1 CONTAINS AN ACTIVATION DOMAIN THAT STIMULATES TRANSCRIPTION ONLY IN A SPECIFIC CONTEXT OF FACTOR-BINDING SITES [J].

GIESE, K ;

GROSSCHEDL, R .

EMBO JOURNAL, 1993, 12 (12) :4667-4676

[16] ASSEMBLY AND FUNCTION OF A TCR-ALPHA ENHANCER COMPLEX IS DEPENDENT ON LEF-1-INDUCED DNA BENDING AND MULTIPLE PROTEIN-PROTEIN INTERACTIONS [J].

GIESE, K ;

KINGSLEY, C ;

KIRSHNER, JR ;

GROSSCHEDL, R .

GENES & DEVELOPMENT, 1995, 9 (08) :995-1008

[17] RELATED TARGET ENHANCERS FOR DORSAL AND NF-KAPPA-B SIGNALING PATHWAYS [J].

GONZALEZCRESPO, S ;

LEVINE, M .

SCIENCE, 1994, 264 (5156) :255-258

[18] HUMAN BETA-INTERFERON GENE-EXPRESSION IS REGULATED BY AN INDUCIBLE ENHANCER ELEMENT [J].

GOODBOURN, S ;

ZINN, K ;

MANIATIS, T .

CELL, 1985, 41 (02) :509-520

[19] EARLY AND LATE PERIODIC PATTERNS OF EVEN SKIPPED EXPRESSION ARE CONTROLLED BY DISTINCT REGULATORY ELEMENTS THAT RESPOND TO DIFFERENT SPATIAL CUES [J].

GOTO, T ;

MACDONALD, P ;

MANIATIS, T .

CELL, 1989, 57 (03) :413-422

[20] HIGHER-ORDER NUCLEOPROTEIN COMPLEXES IN TRANSCRIPTION - ANALOGIES WITH SITE-SPECIFIC RECOMBINATION [J].

GROSSCHEDL, R .

CURRENT OPINION IN CELL BIOLOGY, 1995, 7 (03) :362-370

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