CELL-DIVISION IN HIGHER-PLANTS - A CDC2 GENE, ITS 34-KDA PRODUCT, AND HISTONE-H1 KINASE-ACTIVITY IN PEA

被引：139

作者：

FEILER, HS ^{[1
]}

JACOBS, TW ^{[1
]}

机构：

[1] UNIV ILLINOIS,DEPT PLANT BIOL,URBANA,IL 61801

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1990年 / 87卷 / 14期

关键词：

Cell cycle; Mitosis; Pisum sativum; Polymerase chain reaction; Protein phosphorylation;

D O I：

10.1073/pnas.87.14.5397

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The mitotic cell cycle of yeast and animal cells is regulated by the cdc2 gene and its product, the p34 protein kinase, and by other components of the MPF or histone H1 kinase complex. We present evidence that cdc2, p34, and a histone H1 kinase also exist in higher plants. Protein extracts from 10 plant species surveyed display a 34-kDa component recognized by a monoclonal antibody directed against an evolutionarily conserved epitope of fission yeast p34. Nondenatured protein extracts of mitotic Pisum sativum (garden pea) tissues were fractionated by gel filtration, electrophoretically separated under denaturing conditions, and immunoblotted. p34 crossreactive material was apparent in both low and high molecular mass fractions, indicating that pea p34 occurs as both a monomer and as part of a high molecular mass complex. Histone H1 kinase activity was found predominantly in the higher molecular mass fractions, those to which the least phosphorylated form of pea p34 was confined. We also report the cloning of the pea homologue of cdc2 by polymerase chain reaction. DNA sequence analysis reveals perfect conservation of the hallmark "PSTAIR" sequence motif found in all cdc2 gene products analyzed to date.

引用

页码：5397 / 5401

页数：5

共 41 条

[11] IDENTIFICATION OF P34 AND P13, HUMAN HOMOLOGS OF THE CELL-CYCLE REGULATORS OF FISSION YEAST ENCODED BY CDC2+ AND SUC1+ [J].