A decade of discovery: CRISPR functions and applications

被引：217

作者：

Barrangou, Rodolphe ^{[1
]}

Horvath, Philippe ^{[2
]}

机构：

[1] North Carolina State Univ, Dept Food Bioproc & Nutr Sci, 400 Dan Allen Dr,Campus Box 7624, Raleigh, NC 27695 USA

[2] DuPont Nutr & Hlth, BP10, F-86220 Dange St Romain, France

来源：

NATURE MICROBIOLOGY | 2017年 / 2卷 / 07期

关键词：

BACTERIAL IMMUNE-SYSTEM; RNA-GUIDED ENDONUCLEASE; SEQUENCE-SPECIFIC ANTIMICROBIALS; TARGET DNA RECOGNITION; CAS ADAPTIVE IMMUNITY; LACTIC-ACID BACTERIA; STREPTOCOCCUS-THERMOPHILUS; ESCHERICHIA-COLI; GENE-EXPRESSION; ACQUIRED-RESISTANCE;

D O I：

10.1038/nmicrobiol.2017.92

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

This year marks the tenth anniversary of the identification of the biological function of CRISPR-Cas as adaptive immune systems in bacteria. In just a decade, the characterization of CRISPR-Cas systems has established a novel means of adaptive immunity in bacteria and archaea and deepened our understanding of the interplay between prokaryotes and their environment, and CRISPR-based molecular machines have been repurposed to enable a genome editing revolution. Here, we look back on the historical milestones that have paved the way for the discovery of CRISPR and its function, and discuss the related technological applications that have emerged, with a focus on microbiology. Lastly, we provide a perspective on the impacts the field has had on science and beyond.

引用

页数：9

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