MiRNAs on the move: miRNA biogenesis and the RNAi machinery

被引：311

作者：

Murchison, EP ^{[1
]}

Hannon, GJ ^{[1
]}

机构：

[1] Cold Spring Harbor Lab, Watson Sch Biol Sci, Cold Spring Harbor, NY 11724 USA

来源：

CURRENT OPINION IN CELL BIOLOGY | 2004年 / 16卷 / 03期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/j.ceb.2004.04.003

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Recent advances have led to a more detailed understanding of RNA interference and its role in microRNA biogenesis and function. Primary microRNA transcripts are processed by the RNaselll nuclease, Drosha, and are exported from the nucleus by Exportin-5. Dicer cleaves microRNAs into their mature forms, which can be incorporated into effector complexes that mediate gene silencing activities. The 3' two-nucleotide overhang structure, a signature of RNaselll cleavage, has been identified as a critical specificity determinant in targeting and maintaining small RNAs in the RNA interference pathway. MicroRNA functional analyses and genetic and biochemical interrogation of components of the pathway are starting to provide a glimpse at the range of biological processes and phenomena regulated by RNA interference.

引用

页码：223 / 229

页数：7

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