Genes and mechanisms related to RNA interference regulate expression of the small temporal RNAs that control C-elegans developmental timing

被引：1465

作者：

Grishok, A

Pasquinelli, AE

Conte, D

Li, N

Parrish, S

Ha, I

Baillie, DL

Fire, A

Ruvkun, G

Mello, CC ^{[1
]}

机构：

[1] Univ Massachusetts, Sch Med, Program Mol Med, Worcester, MA 01605 USA

[2] Univ Massachusetts, Sch Med, Howard Hughes Med Inst, Worcester, MA 01605 USA

[3] Massachusetts Gen Hosp, Dept Mol Biol, Boston, MA 02114 USA

[4] Harvard Univ, Sch Med, Dept Genet, Boston, MA 02114 USA

[5] Johns Hopkins Univ, Biol Grad Program, Baltimore, MD 21218 USA

[6] Simon Fraser Univ, Inst Mol Biol & Biochem, Burnaby, BC V5A 1S6, Canada

[7] Carnegie Inst Sci, Baltimore, MD 21210 USA

[8] Hanwha Chem R&D Inst, Daejun 305345, South Korea

来源：

CELL | 2001年 / 106卷 / 01期

关键词：

D O I：

10.1016/S0092-8674(01)00431-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

RNAi is a gene-silencing phenomenon triggered by double-stranded (ds) RNA and involves the generation of 21 to 26 nt RNA segments that guide mRNA destruction. In Caenorhabditis elegans, lin-4 and let-7 encode small temporal RNAs (stRNAs) of 22 nt that regulate stage-specific development. Here we show that inactivation of genes related to RNAi pathway genes, a homolog of Drosophila Dicer (dcr-1), and two homologs of rde-1 (alg-1 and alg-2), cause heterochronic phenotypes similar to lin-4 and let-7 mutations. Further we show that dcr-1, alg-1, and alg-2 are necessary for the maturation and activity of the lin-4 and let-7 stRNAs. Our findings suggest that a common processing machinery generates guide RNAs that mediate both RNAi and endogenous gene regulation.

引用

页码：23 / 34

页数：12

共 42 条

[1] A HIERARCHY OF REGULATORY GENES CONTROLS A LARVA-TO-ADULT DEVELOPMENTAL SWITCH IN C-ELEGANS [J].