Genome analysis with gene expression microarrays

被引：168

作者：

Schena, M

机构：

[1] Department of Biochemistry, Beckman Center, Stanford University Medical Center, Stanford

来源：

BIOESSAYS | 1996年 / 18卷 / 05期

关键词：

D O I：

10.1002/bies.950180513

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Advances in biochemistry, chemistry and engineering have enabled the development of a new gene expression assay. This 'chip-based' approach utilizes microscopic arrays of cDNAs printed on glass as high-density hybridization targets. Fluorescent probe mixtures derived from total cellular messenger RNA (mRNA) hybridize to cognate elements on the array, allowing accurate measurement of the expression of the corresponding genes. Array densities of >1,000 cDNAs per cm(2) enable quantitative expression monitoring of a large number of genes in a single hybridization. A two-color fluorescence detection scheme allows rapid and simultaneous differential expression analysis of independent biological samples. Mass-produced microarrays provide a new tool for genome expression analysis that may revolutionize genetic dissection, drug discovery and human disease diagnostics.

引用

页码：427 / 431

页数：5

共 25 条

[1] METHOD FOR DETECTION OF SPECIFIC RNAS IN AGAROSE GELS BY TRANSFER TO DIAZOBENZYLOXYMETHYL-PAPER AND HYBRIDIZATION WITH DNA PROBES [J].

ALWINE, JC ;

KEMP, DJ ;

STARK, GR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (12) :5350-5354

[2] SIZING AND MAPPING OF EARLY ADENOVIRUS MESSENGER-RNAS BY GEL-ELECTROPHORESIS OF S1 ENDONUCLEASE-DIGESTED HYBRIDS [J].

BERK, AJ ;

SHARP, PA .

CELL, 1977, 12 (03) :721-732

[3] BIOINFORMATICS [J].

BOGUSKI, MS .

CURRENT OPINION IN GENETICS & DEVELOPMENT, 1994, 4 (03) :383-388

[4] WHOLE-GENOME RANDOM SEQUENCING AND ASSEMBLY OF HAEMOPHILUS-INFLUENZAE RD [J].

FLEISCHMANN, RD ;

ADAMS, MD ;

WHITE, O ;

CLAYTON, RA ;

KIRKNESS, EF ;

KERLAVAGE, AR ;

BULT, CJ ;

TOMB, JF ;

DOUGHERTY, BA ;

MERRICK, JM ;

MCKENNEY, K ;

SUTTON, G ;

FITZHUGH, W ;

FIELDS, C ;

GOCAYNE, JD ;

SCOTT, J ;

SHIRLEY, R ;

LIU, LI ;

GLODEK, A ;

KELLEY, JM ;

WEIDMAN, JF ;

PHILLIPS, CA ;

SPRIGGS, T ;

HEDBLOM, E ;

COTTON, MD ;

UTTERBACK, TR ;

HANNA, MC ;

NGUYEN, DT ;

SAUDEK, DM ;

BRANDON, RC ;

FINE, LD ;

FRITCHMAN, JL ;

FUHRMANN, JL ;

GEOGHAGEN, NSM ;

GNEHM, CL ;

MCDONALD, LA ;

SMALL, KV ;

FRASER, CM ;

SMITH, HO ;

VENTER, JC .

SCIENCE, 1995, 269 (5223) :496-512

[5] LIGHT-DIRECTED, SPATIALLY ADDRESSABLE PARALLEL CHEMICAL SYNTHESIS [J].

FODOR, SPA ;

READ, JL ;

PIRRUNG, MC ;

STRYER, L ;

LU, AT ;

SOLAS, D .

SCIENCE, 1991, 251 (4995) :767-773

[6] THE HUMAN GENOME PROJECT - PROSPECTS AND IMPLICATIONS FOR CLINICAL MEDICINE [J].

GREEN, ED ;

WATERSTON, RH .

JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 1991, 266 (14) :1966-1975

[7] DIRECT FLUORESCENCE ANALYSIS OF GENETIC POLYMORPHISMS BY HYBRIDIZATION WITH OLIGONUCLEOTIDE ARRAYS ON GLASS SUPPORTS [J].

GUO, Z ;

GUILFOYLE, RA ;

THIEL, AJ ;

WANG, RF ;

SMITH, LM .

NUCLEIC ACIDS RESEARCH, 1994, 22 (24) :5456-5465

[8] AN INVENTORY OF 1152 EXPRESSED SEQUENCE TAGS OBTAINED BY PARTIAL SEQUENCING OF CDNAS FROM ARABIDOPSIS-THALIANA [J].

HOFTE, H ;

DESPREZ, T ;

AMSELEM, J ;

CHIAPELLO, H ;

ROUZE, P ;

CABOCHE, M ;

MOISAN, A ;

JOURJON, MF ;

CHARPENTEAU, JL ;

BERTHOMIEU, P ;

GUERRIER, D ;

GIRAUDAT, J ;

QUIGLEY, F ;

THOMAS, F ;

YU, DY ;

MACHE, R ;

RAYNAL, M ;

COOKE, R ;

GRELLET, F ;

DELSENY, M ;

PARMENTIER, Y ;

DEMARCILLAC, G ;

GIGOT, C ;

FLECK, J ;

PHILIPPS, G ;

AXELOS, M ;

BARDET, C ;

TREMOUSAYGUE, D ;

LESCURE, B .

PLANT JOURNAL, 1993, 4 (06) :1051-1061

[9] DIAGNOSIS OF CHRONIC MYELOID AND ACUTE LYMPHOCYTIC LEUKEMIAS BY DETECTION OF LEUKEMIA-SPECIFIC MESSENGER-RNA SEQUENCES AMPLIFIED INVITRO [J].

KAWASAKI, ES ;

CLARK, SS ;

COYNE, MY ;

SMITH, SD ;

CHAMPLIN, R ;

WITTE, ON ;

MCCORMICK, FP .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (15) :5698-5702

[10] BIOPOLYMER SYNTHESIS ON POLYPROPYLENE SUPPORTS - OLIGONUCLEOTIDE ARRAYS [J].

MATSON, RS ;

RAMPAL, J ;

PENTONEY, SL ;

ANDERSON, PD ;

COASSIN, P .

ANALYTICAL BIOCHEMISTRY, 1995, 224 (01) :110-116

← 1 2 3 →