栝楼桂枝颗粒对脑缺血再灌注损伤大鼠的血脑屏障通透性及神经保护作用

被引：15

作者：

张玉琴 ^{[1
]}

李煌 ^{[2
]}

许文 ^{[2
]}

徐伟 ^{[2
]}

黄枚 ^{[2
]}

褚克丹 ^{[2
]}

陈立典 ^{[3
]}

机构：

[1] 福建中医药大学中西医结合研究院

[2] 福建中医药大学药学院

[3] 福建中医药大学康复医学院

来源：

中华中医药杂志 | 2015年 / 30卷 / 05期

关键词：

栝楼桂枝颗粒; 血脑屏障; 脑缺血再灌注损伤; HPLC-MS/MS; 神经保护;

D O I：

暂无

中图分类号：

R285.5 [中药实验药理];

学科分类号：

1008 ;

摘要：

目的:探讨栝楼桂枝颗粒(GLGZG)对脑缺血再灌注损伤大鼠的血脑屏障(BBB)通透性的影响及神经保护作用。方法:文章采用大脑中动脉栓线阻断法制备局灶性脑缺再灌注损伤模型(MCAO),缺血2h后进行再灌注。采用HPLC-MS/MS定性分析正常大鼠及MCAO模型大鼠血浆、脑组织及脑脊液中GLGZG的成分。同时观察MCAO模型大鼠的神经功能缺损、BBB通透性变化、脑梗死体积测定和病理组织学变化。结果:经HPLC-MS/MS定性分析得出,GLGZG中的成分瓜氨酸、芍药内酯苷、芍药苷、芹糖甘草苷、甘草苷可以通过BBB进入脑组织中,而在模型组中,成分异甘草素以及甘草酸亦可在脑组织或脑脊液中检测到。同时GLGZG能降低MCAO模型大鼠的神经功能缺损、减少伊文思蓝(EB)的渗透量、缩小脑梗死体积、减轻脑缺血再灌注的病理组织损伤。结论:GLGZG中的成分瓜氨酸、芍药内酯苷、芍药苷、芹糖甘草苷、甘草苷、芹糖异甘草苷、异甘草苷、甘草素、异甘草素以及甘草酸可以透过BBB进入脑组织中,对脑缺血再灌注损伤的大鼠起到神经保护作用,为今后进一步研究及临床用药提供实验依据。

引用

页码：1410 / 1417

页数：8

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