PERINATAL ACTIVATION OF A TYROSINE AMINOTRANSFERASE FUSION GENE DOES NOT OCCUR IN ALBINO LETHAL MICE

被引：19

作者：

BEERMANN, F ^{[1
]}

HUMMLER, E ^{[1
]}

SCHMID, E ^{[1
]}

SCHUTZ, G ^{[1
]}

机构：

[1] GERMAN CANC RES CTR,INST CELL & TUMOR BIOL,DIV MOLEC BIOL CELL 1,NEUENHEIMER FELD 280,W-6900 HEIDELBERG,GERMANY

来源：

MECHANISMS OF DEVELOPMENT | 1993年 / 42卷 / 1-2期

关键词：

ALBINO-DELETION COMPLEX; LIVER-SPECIFIC GENE EXPRESSION; PERINATAL GENE ACTIVATION; TRANSGENIC MOUSE; TYROSINE AMINOTRANSFERASE;

D O I：

10.1016/0925-4773(93)90098-I

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

To investigate developmental expression of the rat tyrosine aminotransferase (TAT) gene in normal and in albino lethal mice we generated transgenic mice carrying a fusion gene of TAT 5'-sequences (11 kb) and the bacterial chloramphenicol acetyltransferase (CAT) gene. In four lines, CAT activity was found only in liver. RNA analyses on a high-expressing line showed that transgenic expression follows expression of mouse TAT mRNA: it is inducible by glucocorticoids and activated perinatally. This perinatal activation of transgene expression does not occur in lethal albino mice (c14CoS/c14CoS) which are characterized by reduced mRNA levels of several liver-specific enzymes involved in gluconeogenesis. In conclusion, the data show that the 5'-flanking region of the rat TAT gene contains elements specifying regulated expression and establish that the 5'-flanking region of the TAT gene is responsive to the enzyme deficiency characteristic of the albino lethal mice.

引用

页码：59 / 65

页数：7

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