PREF-1, A PROTEIN CONTAINING EGF-LIKE REPEATS, INHIBITS ADIPOCYTE DIFFERENTIATION

被引:572
作者
SMAS, CM
SUL, HS
机构
[1] Department of Nutrition Harvard School of Public Health Boston
基金
美国国家卫生研究院;
关键词
D O I
10.1016/0092-8674(93)90252-L
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
With the aim of identifying novel regulators of adipocyte differentiation, we have cloned and characterized preadipocyte factor 1 (pref-1), a novel member of the epidermal growth factor (EGF)-like family of proteins. Pref-1 is synthesized as a transmembrane protein with six tandem EGF-like repeats. In preadipocytes, multiple discrete forms of pref-1 protein of 45-60 kd are present, owing in part to N-linked glycosylation. While pref-1 mRNA is abundant in preadipocytes, its expression is completely abolished during differentiation of 3T3-L1 preadipocytes to adipocytes. Moreover, constitutive expression of pref-1 in preadipocytes, which in effect blocks its down-regulation, drastically inhibits adipose differentiation. This indicates that pref-1 functions asa negative regulator of adipocyte differentiation, possibly in a manner analogous to EGF-like proteins that govern cell fate decisions in invertebrates.
引用
收藏
页码:725 / 734
页数:10
相关论文
共 75 条
[1]  
ARATANI Y, 1988, J BIOL CHEM, V263, P16163
[2]   MAMMALIAN SUBTILISINS - THE LONG-SOUGHT DIBASIC PROCESSING ENDOPROTEASES [J].
BARR, PJ .
CELL, 1991, 66 (01) :1-3
[3]  
BERNLOHR DA, 1985, J BIOL CHEM, V260, P5563
[4]   ENDOTHELIAL LEUKOCYTE ADHESION MOLECULE-1 - AN INDUCIBLE RECEPTOR FOR NEUTROPHILS RELATED TO COMPLEMENT REGULATORY PROTEINS AND LECTINS [J].
BEVILACQUA, MP ;
STENGELIN, S ;
GIMBRONE, MA ;
SEED, B .
SCIENCE, 1989, 243 (4895) :1160-1165
[5]   TISSUE-SPECIFIC EXPRESSION, DEVELOPMENTAL REGULATION, AND GENETIC-MAPPING OF THE GENE ENCODING CCAAT ENHANCER BINDING-PROTEIN [J].
BIRKENMEIER, EH ;
GWYNN, B ;
HOWARD, S ;
JERRY, J ;
GORDON, JI ;
LANDSCHULZ, WH ;
MCKNIGHT, SL .
GENES & DEVELOPMENT, 1989, 3 (08) :1146-1156
[6]   SIMILARITY BETWEEN CELL-CYCLE GENES OF BUDDING YEAST AND FISSION YEAST AND THE NOTCH GENE OF DROSOPHILA [J].
BREEDEN, L ;
NASMYTH, K .
NATURE, 1987, 329 (6140) :651-654
[7]   MURINE EPIDERMAL GROWTH-FACTOR - STRUCTURE AND FUNCTION [J].
BURGESS, AW ;
LLOYD, CJ ;
SMITH, S ;
STANLEY, E ;
WALKER, F ;
FABRI, L ;
SIMPSON, RJ ;
NICE, EC .
BIOCHEMISTRY, 1988, 27 (14) :4977-4985
[8]  
CALVO JC, 1991, CAMPJ, V266, P11237
[9]  
CARPENTER G, 1990, J BIOL CHEM, V265, P7709
[10]   ISOLATION OF BIOLOGICALLY-ACTIVE RIBONUCLEIC-ACID FROM SOURCES ENRICHED IN RIBONUCLEASE [J].
CHIRGWIN, JM ;
PRZYBYLA, AE ;
MACDONALD, RJ ;
RUTTER, WJ .
BIOCHEMISTRY, 1979, 18 (24) :5294-5299