ISOLATION OF BIOLOGICALLY-ACTIVE RIBONUCLEIC-ACID FROM SOURCES ENRICHED IN RIBONUCLEASE

被引：23762

作者：

CHIRGWIN, JM ^{[1
]}

PRZYBYLA, AE ^{[1
]}

MACDONALD, RJ ^{[1
]}

RUTTER, WJ ^{[1
]}

机构：

[1] UNIV CALIF SAN FRANCISCO,DEPT BIOCHEM & BIOPHYS,SAN FRANCISCO,CA 94143

来源：

BIOCHEMISTRY | 1979年 / 18卷 / 24期

关键词：

D O I：

10.1021/bi00591a005

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Intact ribonucleic acid (RNA) has been prepared from tissues rich in ribonuclease such as the rat pancreas by efficient homogenization in a 4 M solution of the potent protein denaturant guanidinium thiocyanate plus 0.1 M 2-mercaptoethanol to break protein disulfide bonds. The RNA was iso lated free of protein by ethanol precipitation or by sedimentation through cesium chloride. Rat pancreas RNA obtained by these means has been used as a source for the purification of α-amylase messenger ribonucleic acid. © 1979, American Chemical Society. All rights reserved.

引用

页码：5294 / 5299

页数：6

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