PURIFICATION OF RAT LIVER RNASE INHIBITOR AND ITS EFFECT ON POLYRIBOSOME INTEGRITY

被引：122

作者：

GRIBNAU, AAM

SCHOENMAKERS, JG

BLOEMENDAL, H

机构：

[1] Department of Biochemistry, University of Nijmegen, Nijmegen

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1969年 / 130卷 / 1-2期

关键词：

D O I：

10.1016/0003-9861(69)90007-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The RNase inhibitor from 100,000 g rat liver supernatant fraction has been purified 3000-4000-fold by ammonium sulfate fractionation, DEAE-Sephadex A-50 chromatography, and gel filtration on Sephadex G-100. Purification of the inhibitor is paralleled by a concomitant purification of the factor which stabilizes the polysomal profile on sucrose gradients. This is also reflected by amino acid incorporation ability of the polyribosomes. Increasing purity of the factor is associated with increasing lability. © 1969.

引用

收藏

页码：48 / +

页数：1

相关论文

共 18 条

[1] ESTIMATION OF MOLECULAR WEIGHTS OF PROTEINS BY SEPHADEX GEL-FILTRATION [J].

ANDREWS, P .

BIOCHEMICAL JOURNAL, 1964, 91 (02) :222-+

[2] RELATION OF RIBONUCLEASE AND RIBONUCLEASE INHIBITOR TO ISOLATION OF POLYSOMES FROM RAT LIVER [J].

BLOBEL, G ;

POTTER, VR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1966, 55 (05) :1283-&

[3]

BLOEMEND.H, 1966, CANCER RES, V26, P1497

[4] PREPARATION OF RAT-LIVER POLYSOMES WITHOUT UTILIZATION OF DETERGENT [J].

BLOEMENDAL, H ;

BENEDETTI, EL ;

BONT, WS .

BIOCHIMICA ET BIOPHYSICA ACTA, 1964, 87 (01) :177-&

[5]

BLOEMENDAL H, 1963, ZONE ELECTROPHORESIS

[6] STABILIZING EFFECT OF SUPERNATANT FRACTION ON STRUCTURE OF POLYRIBOSOMES FROM RAT LIVER [J].

BONT, WS ;

REZELMAN, C ;

BLOEMENDAL, H .

BIOCHEMICAL JOURNAL, 1965, 95 (02) :C15-+

[7] STUDIES ON CYTOPLASMIC RIBONUCLEIC ACID FROM RAT LIVER .8. STABILITY OF POLYRIBOSOMES FROM NORMAL AND REGENERATING RAT LIVER [J].

BONT, WS ;

REZELMAN, G ;

MEISNER, I ;

BLOEMENDAL, H .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1967, 119 (1-3) :36-+

[8] POLYRIBOSOMES IN EXTRACTS OF BONE MARROW ERYTHROID CELLS AND THEIR PRESERVATION BY HEPATIC RIBONUCLEASE INHIBITOR [J].

GRAU, O ;

FAVELUKES, G .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1968, 125 (02) :647-+

[9] STABILIZATION OF RAT LIVER POLYRIBOSOMES BY ENDOGENOUS RIBONUCLEASE INHIBITOR [J].

LAWFORD, GR ;

SCHACHTER, H .

CANADIAN JOURNAL OF BIOCHEMISTRY, 1967, 45 (01) :144-+

[10]

LOWRY OH, 1951, J BIOL CHEM, V193, P265