DETECTION OF A CYTOSOLIC GLUTAMINE-SYNTHETASE IN LEAVES OF NICOTIANA-TABACUM-L BY IMMUNOCYTOCHEMICAL METHODS

被引：72

作者：

CARVALHO, H ^{[1
]}

PEREIRA, S ^{[1
]}

SUNKEL, C ^{[1
]}

SALEMA, R ^{[1
]}

机构：

[1] UNIV PORTO, INST BOT, P-4100 OPORTO, PORTUGAL

来源：

PLANT PHYSIOLOGY | 1992年 / 100卷 / 03期

关键词：

D O I：

10.1104/pp.100.3.1591

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Two glutamine synthetase (GS) polypeptides (44 and 39 kD) were immunodetected on western blots of leaf extracts from tobacco (Nicotiana tabacum L.), a plant that has been reported to contain only chloroplast GS in the leaves. By immunocytochemical methods, we confirmed the localization of GS in the cytosol of cells in the vascular tissue and in the chloroplasts of mesophyll cells.

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页码：1591 / 1594

页数：4

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