IMMUNOLOGICAL STUDIES ON GLUTAMINE-SYNTHETASE USING ANTISERA RAISED TO THE 2 PLANT-FORMS OF THE ENZYME FROM PHASEOLUS ROOT-NODULES

Antisera specific for glutamine synthetase (GS) well raised to the 2 forms of the enzyme from the plant fraction of root nodules of P. vulgaris. The 2 antisera recognized both forms of plant nodule GS and also the enzyme from some other higher plant [Pisum sativum, Glycine max] tissues. The antiserum did not cross-react with GS from free-living or bacteroid Rhizobium phaseoli nor with the enzyme from representatives of green algae, fungi, mammals and bacteria [Chlamydomonas reinhardii, Candida spp., ovine brain and Escherichia coli, respectively]. One of the forms of nodule GS may be closely related to the root enzyme whereas the other, the nodule specific form, has some antigenic differences.