GENOMIC ORGANIZATION AND FULL-LENGTH CDNA SEQUENCE OF HUMAN COLLAGEN-X

被引：50

作者：

REICHENBERGER, E

BEIER, F

LUVALLE, P

OLSEN, BR

VONDERMARK, K

BERTLING, WM

机构：

[1] UNIV ERLANGEN NURNBERG, MED CLIN 3,MAX PLANCK SOC,CLIN RES UNIT RHEUMATOL, SCHWABACHANLAGE 10, W-8520 ERLANGEN, GERMANY

[2] HARVARD UNIV, SCH MED, DEPT ANAT & CELLULAR BIOL, BOSTON, MA 02115 USA

来源：

FEBS LETTERS | 1992年 / 311卷 / 03期

关键词：

HUMAN CARTILAGE; COLLAGEN; HUMAN TYPE-X; NUCLEOTIDE SEQUENCE; REGULATORY SEQUENCE; EVOLUTION;

D O I：

10.1016/0014-5793(92)81126-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have determined the full-length cDNA sequence of the human alpha1(X) collagen gene by sequence analysis of a genomic clone ERG [(1991) Dev. Biol. 148, 562-572], and of cDNA fragments generated from a reverse transcribed as alpha1(X) mRNA by PCR. We defined the promoter region, the transcription initiation site and the full-length 5'-untranslated region. We also report the exon/intron boundaries of the transcript and the complete 3'-untranslated region as well as a 3'-flanking sequence containing two additional polyadenylation signals. The promoter region is homologous to chicken and mouse type X promoters within several highly conserved regions. The genomic organization shows high homologies to chicken and mouse.

引用

页码：305 / 310

页数：6

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