PURIFICATION AND CHARACTERIZATION OF BOVINE BRAIN TYPE-I PHOSPHATIDYLINOSITOL KINASE

被引：119

作者：

MORGAN, SJ

SMITH, AD

PARKER, PJ

机构：

[1] LUDWIG INST CANC RES,91 RIDING HOUSE ST,LONDON W1P 8BT,ENGLAND

[2] UNIV COLL & MIDDLESEX SCH MED,DEPT CHEM PATHOL,LONDON,ENGLAND

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1990年 / 191卷 / 03期

关键词：

D O I：

10.1111/j.1432-1033.1990.tb19185.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Investigation into the phosphatidylinositol kinase activities in bovine brain has revealed the presence of a type I PtdIns kinase activity. This classification is based upon potent inhibition by neutral detergent and the production of a phosphatidylinositol phosphate that can be distinguished from phosphatidyl‐inositol 4‐phosphate [PtdIns(4)P] by thin‐layer chromatography. The enzyme has been substantially purified and the activity is associated with an 85‐kDa polypeptide on SDS/polyacrylamide gel electrophoresis. Analysis of the product confirms the identification of the enzyme as a type I PtdIns kinase. The purified kinase has been characterized with respect to substrate dependence (Mg2+, ATP, PtdIns), substrate presentation (pure lipid versus mixed micelle) and specificity [PtdIns versus PtdIns(4)P and phosphatidylinositol 4,5‐bisphosphate]. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

收藏

页码：761 / 767

页数：7

相关论文

共 31 条

[21] PURIFICATION OF PHOSPHATIDYLINOSITOL KINASE FROM BOVINE BRAIN MYELIN [J].

SALTIEL, AR ;

FOX, JA ;

SHERLINE, P ;

SAHYOUN, N ;

CUATRECASAS, P .

BIOCHEMICAL JOURNAL, 1987, 241 (03) :759-763

[22] METABOLIC AND STRUCTURAL EVIDENCE FOR THE EXISTENCE OF A 3RD-SPECIES OF POLYPHOSPHOINOSITIDE IN CELLS - D-PHOSPHATIDYL-MYO-INOSITOL 3-PHOSPHATE [J].

STEPHENS, L ;

HAWKINS, PT ;

DOWNES, CP .

BIOCHEMICAL JOURNAL, 1989, 259 (01) :267-276

[23] RELEASE OF CA-2+ FROM A NONMITOCHONDRIAL INTRACELLULAR STORE IN PANCREATIC ACINAR-CELLS BY INOSITOL-1,4,5-TRISPHOSPHATE [J].

STREB, H ;

IRVINE, RF ;

BERRIDGE, MJ ;

SCHULZ, I .

NATURE, 1983, 306 (5938) :67-69

[24] EVIDENCE THAT THE ROUS-SARCOMA VIRUS TRANSFORMING GENE-PRODUCT PHOSPHORYLATES PHOSPHATIDYLINOSITOL AND DIACYLGLYCEROL [J].

SUGIMOTO, Y ;

WHITMAN, M ;

CANTLEY, LC ;

ERIKSON, RL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (07) :2117-2121

[25] UNSATURATED DIACYLGLYCEROL AS A POSSIBLE MESSENGER FOR THE ACTIVATION OF CALCIUM-ACTIVATED, PHOSPHOLIPID DEPENDENT PROTEIN-KINASE SYSTEM [J].

TAKAI, Y ;

KISHIMOTO, A ;

KIKKAWA, U ;

MORI, T ;

NISHIZUKA, Y .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1979, 91 (04) :1218-1224

[26] AN INOSITOL TETRAKISPHOSPHATE-CONTAINING PHOSPHOLIPID IN ACTIVATED NEUTROPHILS [J].

TRAYNORKAPLAN, AE ;

HARRIS, AL ;

THOMPSON, BL ;

TAYLOR, P ;

SKLAR, LA .

NATURE, 1988, 334 (6180) :353-356

[27] THE COLONY STIMULATING FACTOR-I RECEPTOR ASSOCIATES WITH AND ACTIVATES PHOSPHATIDYLINOSITOL-3 KINASE [J].

VARTICOVSKI, L ;

DRUKER, B ;

MORRISON, D ;

CANTLEY, L ;

ROBERTS, T .

NATURE, 1989, 342 (6250) :699-702

[28] PURIFICATION AND CHARACTERIZATION OF A PHOSPHATIDYLINOSITOL KINASE FROM A431 CELLS [J].

WALKER, DH ;

DOUGHERTY, N ;

PIKE, LJ .

BIOCHEMISTRY, 1988, 27 (17) :6504-6511

[29] EVIDENCE FOR 2 DISTINCT PHOSPHATIDYLINOSITOL KINASES IN FIBROBLASTS - IMPLICATIONS FOR CELLULAR-REGULATION [J].

WHITMAN, M ;

KAPLAN, D ;

ROBERTS, T ;

CANTLEY, L .

BIOCHEMICAL JOURNAL, 1987, 247 (01) :165-174

[30] TYPE-I PHOSPHATIDYLINOSITOL KINASE MAKES A NOVEL INOSITOL PHOSPHOLIPID, PHOSPHATIDYLINOSITOL-3-PHOSPHATE [J].

WHITMAN, M ;

DOWNES, CP ;

KEELER, M ;

KELLER, T ;

CANTLEY, L .

NATURE, 1988, 332 (6165) :644-646

← 1 2 3 4 →