PURIFICATION AND CHARACTERIZATION OF BOVINE BRAIN TYPE-I PHOSPHATIDYLINOSITOL KINASE

被引：119

作者：

MORGAN, SJ

SMITH, AD

PARKER, PJ

机构：

[1] LUDWIG INST CANC RES,91 RIDING HOUSE ST,LONDON W1P 8BT,ENGLAND

[2] UNIV COLL & MIDDLESEX SCH MED,DEPT CHEM PATHOL,LONDON,ENGLAND

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1990年 / 191卷 / 03期

关键词：

D O I：

10.1111/j.1432-1033.1990.tb19185.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Investigation into the phosphatidylinositol kinase activities in bovine brain has revealed the presence of a type I PtdIns kinase activity. This classification is based upon potent inhibition by neutral detergent and the production of a phosphatidylinositol phosphate that can be distinguished from phosphatidyl‐inositol 4‐phosphate [PtdIns(4)P] by thin‐layer chromatography. The enzyme has been substantially purified and the activity is associated with an 85‐kDa polypeptide on SDS/polyacrylamide gel electrophoresis. Analysis of the product confirms the identification of the enzyme as a type I PtdIns kinase. The purified kinase has been characterized with respect to substrate dependence (Mg2+, ATP, PtdIns), substrate presentation (pure lipid versus mixed micelle) and specificity [PtdIns versus PtdIns(4)P and phosphatidylinositol 4,5‐bisphosphate]. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：761 / 767

页数：7

共 31 条

[1] PDGF-DEPENDENT TYROSINE PHOSPHORYLATION STIMULATES PRODUCTION OF NOVEL POLYPHOSPHOINOSITIDES IN INTACT-CELLS [J].