Cloning by Polymerase Chain Reaction of a New Mouse TGF-beta, mTGF-beta 3

被引：53

作者：

Denhez, Fabienne ^{[1
]}

Lafyatis, Robert ^{[1
]}

Kondaiah, Paturu ^{[1
]}

Roberts, Anita B. ^{[1
]}

Sporn, Michael B. ^{[1
]}

机构：

[1] NIH, Lab Chemoprevent, Bldg 41,Room C629, Bethesda, MD 20892 USA

来源：

GROWTH FACTORS | 1990年 / 3卷 / 02期

关键词：

TGF-beta; PCR; development;

D O I：

10.3109/08977199009108276

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Two TGF-beta s, TGF-beta I and 2, have previously been isolated from the mouse. Here we report the isolation of a murine TGF-beta 3 cDNA. RNAs extracted from 15-day-old mouse embryos and several mouse cell lines were reverse-transcribed. These cDNA mixtures were used as substrates for polymerase chain-reaction amplifications, using oligonucleotides designed on the basis of known human and chicken TGF-beta 3 sequences, including the initiation and stop codons. Several overlapping cDNAs containing either the amino-terminal domain or the carboxy-terminal domain, as well as the complete 1.2-kb coding region of the mouse TGF-beta 3 cDNA were obtained. The mouse TGF-beta 3 coding region is 1230 nucleotides long and codes for a 410 amino acid polypeptide very similar to its human counterpart. This cDNA hybridizes to a unique 3.5-kb RNA and is differentially expressed in various mouse tissues and at different embryonic stages.

引用

页码：139 / 146

页数：8

共 21 条

[11] PATTERNS OF EXPRESSION OF MURINE VGR-1 AND BMP-2A RNA SUGGEST THAT TRANSFORMING GROWTH FACTOR-BETA-LIKE GENES COORDINATELY REGULATE ASPECTS OF EMBRYONIC-DEVELOPMENT [J].

LYONS, KM ;

PELTON, RW ;

HOGAN, BLM .

GENES & DEVELOPMENT, 1989, 3 (11) :1657-1668

[12] THE TGF-BETA FAMILY OF GROWTH AND DIFFERENTIATION FACTORS [J].

MASSAGUE, J .

CELL, 1987, 49 (04) :437-438

[13] MURINE TRANSFORMING GROWTH FACTOR-BETA-2 CDNA SEQUENCE AND EXPRESSION IN ADULT TISSUES AND EMBRYOS [J].

MILLER, DA ;

LEE, A ;

PELTON, RW ;

CHEN, EY ;

MOSES, HL ;

DERYNCK, R .

MOLECULAR ENDOCRINOLOGY, 1989, 3 (07) :1108-1114

[14] A TRANSCRIPT FROM A DROSOPHILA PATTERN GENE PREDICTS A PROTEIN HOMOLOGOUS TO THE TRANSFORMING GROWTH-FACTOR-BETA FAMILY [J].

PADGETT, RW ;

STJOHNSTON, RD ;

GELBART, WM .

NATURE, 1987, 325 (6099) :81-84

[15]

ROBERTS AB, 1988, RECENT PROG HORM RES, V44, P157

[16] ARG-GLY-ASP - A VERSATILE CELL RECOGNITION SIGNAL [J].

RUOSLAHTI, E ;

PIERSCHBACHER, MD .

CELL, 1986, 44 (04) :517-518

[17] DNA SEQUENCING WITH CHAIN-TERMINATING INHIBITORS [J].

SANGER, F ;

NICKLEN, S ;

COULSON, AR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (12) :5463-5467

[18]

Spom M.B., 1987, J CELL BIOL, V105, P1039

[19]

TENDIJKE P, 1988, P NATL ACAD SCI USA, V85, P4715

[20] Transforming Growth Factor Beta-1 in Acute Myocardial Infarction in Rats [J].

Thompson, Nancy L. ;

Bazoberry, Fernando ;

Speir, Edith H. ;

Casscells, Ward ;

Ferrans, Victor J. ;

Flanders, Kathleen C. ;

Kondaiah, Patljru ;

Geiser, Andrew G. ;

Sporn, Michael B. .

GROWTH FACTORS, 1988, 1 (01) :91-99

← 1 2 3 →