Cloning by Polymerase Chain Reaction of a New Mouse TGF-beta, mTGF-beta 3

被引：53

作者：

Denhez, Fabienne ^{[1
]}

Lafyatis, Robert ^{[1
]}

Kondaiah, Paturu ^{[1
]}

Roberts, Anita B. ^{[1
]}

Sporn, Michael B. ^{[1
]}

机构：

[1] NIH, Lab Chemoprevent, Bldg 41,Room C629, Bethesda, MD 20892 USA

来源：

GROWTH FACTORS | 1990年 / 3卷 / 02期

关键词：

TGF-beta; PCR; development;

D O I：

10.3109/08977199009108276

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Two TGF-beta s, TGF-beta I and 2, have previously been isolated from the mouse. Here we report the isolation of a murine TGF-beta 3 cDNA. RNAs extracted from 15-day-old mouse embryos and several mouse cell lines were reverse-transcribed. These cDNA mixtures were used as substrates for polymerase chain-reaction amplifications, using oligonucleotides designed on the basis of known human and chicken TGF-beta 3 sequences, including the initiation and stop codons. Several overlapping cDNAs containing either the amino-terminal domain or the carboxy-terminal domain, as well as the complete 1.2-kb coding region of the mouse TGF-beta 3 cDNA were obtained. The mouse TGF-beta 3 coding region is 1230 nucleotides long and codes for a 410 amino acid polypeptide very similar to its human counterpart. This cDNA hybridizes to a unique 3.5-kb RNA and is differentially expressed in various mouse tissues and at different embryonic stages.

引用

页码：139 / 146

页数：8

共 21 条

[1] TRANSFER OF PROTEINS ACROSS MEMBRANES .1. PRESENCE OF PROTEOLYTICALLY PROCESSED AND UNPROCESSED NASCENT IMMUNOGLOBULIN LIGHT-CHAINS ON MEMBRANE-BOUND RIBOSOMES OF MURINE MYELOMA [J].

BLOBEL, G ;

DOBBERSTEIN, B .

JOURNAL OF CELL BIOLOGY, 1975, 67 (03) :835-851

[2] GENOMIC SEQUENCING [J].

CHURCH, GM ;

GILBERT, W .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (07) :1991-1995

[3]

DAVIS LG, 1986, BASIC METHODS MOL BI, P143

[4]

DERYNCK R, 1986, J BIOL CHEM, V261, P4377

[5] A NEW TYPE OF TRANSFORMING GROWTH FACTOR-BETA, TGF-BETA-3 [J].

DERYNCK, R ;

LINDQUIST, PB ;

LEE, A ;

WEN, D ;

TAMM, J ;

GRAYCAR, JL ;

RHEE, L ;

MASON, AJ ;

MILLER, DA ;

COFFEY, RJ ;

MOSES, HL ;

CHEN, EY .

EMBO JOURNAL, 1988, 7 (12) :3737-3743

[6] ROLE OF TRANSFORMING GROWTH FACTOR-BETA IN THE DEVELOPMENT OF THE MOUSE EMBRYO [J].

HEINE, UI ;

MUNOZ, EF ;

FLANDERS, KC ;

ELLINGSWORTH, LR ;

LAM, HYP ;

THOMPSON, NL ;

ROBERTS, AB ;

SPORN, MB .

JOURNAL OF CELL BIOLOGY, 1987, 105 (06) :2861-2876

[7] COMPLEMENTARY DEOXYRIBONUCLEIC-ACID CLONING OF A NOVEL TRANSFORMING GROWTH FACTOR-BETA MESSENGER RIBONUCLEIC-ACID FROM CHICK-EMBRYO CHONDROCYTES [J].

JAKOWLEW, SB ;

DILLARD, PJ ;

KONDAIAH, P ;

SPORN, MB ;

ROBERTS, AB .

MOLECULAR ENDOCRINOLOGY, 1988, 2 (08) :747-755

[8]

KONDAIAH P, 1990, J BIOL CHEM, V265, P1089

[9]

LEHNERT SA, 1988, DEVELOPMENT, V104, P263

[10] VGR-1, A MAMMALIAN GENE RELATED TO XENOPUS VG-1, IS A MEMBER OF THE TRANSFORMING GROWTH FACTOR-BETA GENE SUPERFAMILY [J].

LYONS, K ;

GRAYCAR, JL ;

LEE, A ;

HASHMI, S ;

LINDQUIST, PB ;

CHEN, EY ;

HOGAN, BLM ;

DERYNCK, R .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (12) :4554-4558

← 1 2 3 →