CDNA CLONING AND SEQUENCING FOR THE IMPORT PRECURSOR OF COUPLING FACTOR-6 IN H+-ATP SYNTHASE FROM RAT-LIVER MITOCHONDRIA

被引：13

作者：

HIGUTI, T

OSAKA, F

YOSHIHARA, Y

TSURUMI, C

KAWAMURA, Y

TANI, I

TODA, H

KAKUNO, T

SAKIYAMA, F

TANAKA, K

ICHIHARA, A

机构：

[1] OSAKA UNIV,INST PROT RES,DIV PROT CHEM,SUITA,OSAKA 565,JAPAN

[2] OSAKA UNIV,INST PROT RES,DIV ENZYMOL,SUITA,OSAKA 565,JAPAN

[3] UNIV TOKUSHIMA,INST ENZYME RES,TOKUSHIMA 770,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 171卷 / 03期

基金：

日本学术振兴会;

关键词：

D O I：

10.1016/0006-291X(90)90794-N

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The nucleotide sequence of the import precursor of coupling factor 6 (factor 6) of rat liver H+-ATP synthase has been determined from a recombinant cDNA clone isolated by screening a rat liver cDNA library with a probe DNA. The sequence was composed of 458 nucleotides including a coding region for the import precursor of factor 6 and noncoding regions of both the 5′- and 3′-sides. The import precursor of factor 6 and its mature polypeptide deduced from the open reading frame consisted of 108 and 76 amino acid residues with a molecular weight of 12,494 and 8,927, respectively. The presequence of 32 amino acids could be the import signal peptide which serves to direct the protein into the mitochondrial matrix. © 1990.

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页码：1079 / 1086

页数：8

相关论文

共 40 条

[1] PURIFICATION OF BIOLOGICALLY-ACTIVE GLOBIN MESSENGER-RNA BY CHROMATOGRAPHY ON OLIGOTHYMIDYLIC-ACID-CELLULOSE [J].

AVIV, H ;

LEDER, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1972, 69 (06) :1408-&

[2] STUDIES ON ATPASE COMPLEX FROM BEEF-HEART MITOCHONDRIA .1. ISOLATION AND CHARACTERIZATION OF AN OLIGOMYCIN-SENSITIVE AND AN OLIGOMYCIN-INSENSITIVE ATPASE COMPLEX FROM BEEF-HEART MITOCHONDRIA [J].

BERDEN, JA ;

VOORNBROUWER, MM .

BIOCHIMICA ET BIOPHYSICA ACTA, 1978, 501 (03) :424-439

[3] ISOLATION OF BIOLOGICALLY-ACTIVE RIBONUCLEIC-ACID FROM SOURCES ENRICHED IN RIBONUCLEASE [J].

CHIRGWIN, JM ;

PRZYBYLA, AE ;

MACDONALD, RJ ;

RUTTER, WJ .

BIOCHEMISTRY, 1979, 18 (24) :5294-5299

[4] ISOLATION OF THE MITOCHONDRIAL F1-F0 ADENOSINE-TRIPHOSPHATASE BY SEPHAROSE-HEXYLAMMONIUM CHROMATOGRAPHY - PROPERTIES AND RECONSTITUTION IN LIPOSOMES [J].

DREYFUS, G ;

CELIS, H ;

RAMIREZ, J .

ANALYTICAL BIOCHEMISTRY, 1984, 142 (01) :215-220

[5] AMINO-ACID-SEQUENCE OF BOVINE HEART COUPLING FACTOR-6 [J].

FANG, JK ;

JACOBS, JW ;

KANNER, BI ;

RACKER, E ;

BRADSHAW, RA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (21) :6603-6607

[6] MOLECULAR-CLONING OF CDNA FOR PROTEASOMES (MULTICATALYTIC PROTEINASE COMPLEXES) FROM RAT-LIVER - PRIMARY STRUCTURE OF THE LARGEST COMPONENT (C2) [J].

FUJIWARA, T ;

TANAKA, K ;

KUMATORI, A ;

SHIN, S ;

YOSHIMURA, T ;

ICHIHARA, A ;

TOKUNAGA, F ;

ARUGA, R ;

IWANAGA, S ;

KAKIZUKA, A ;

NAKANISHI, S .

BIOCHEMISTRY, 1989, 28 (18) :7332-7340

[7]

FUTAI M, 1989, ANNU REV BIOCHEM, V58, P111

[8] RESOLUTION AND RECONSTITUTION OF COMPLEX-V OF THE MITOCHONDRIAL OXIDATIVE-PHOSPHORYLATION SYSTEM - PROPERTIES AND COMPOSITION OF THE MEMBRANE SECTOR [J].

GALANTE, YM ;

WONG, SY ;

HATEFI, Y .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1981, 211 (02) :643-651

[9] CODON CATALOG USAGE AND THE GENOME HYPOTHESIS [J].

GRANTHAM, R ;

GAUTIER, C ;

GOUY, M ;

MERCIER, R ;

PAVE, A .

NUCLEIC ACIDS RESEARCH, 1980, 8 (01) :R49-R62

[10] PLASMID SCREENING AT HIGH COLONY DENSITY [J].

HANAHAN, D ;

MESELSON, M .

GENE, 1980, 10 (01) :63-67

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