CHARACTERIZING TRANSITION-STATES IN PROTEIN-FOLDING - AN ESSENTIAL STEP IN THE PUZZLE

被引：279

作者：

FERSHT, AR

机构：

来源：

CURRENT OPINION IN STRUCTURAL BIOLOGY | 1995年 / 5卷 / 01期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1016/0959-440X(95)80012-P

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Transition states in protein folding are being analyzed experimentally and by computer simulation to reveal the sequence of events in the folding and unfolding of proteins at high resolution. Results of this analysis have enabled the reconstruction of folding pathways in vitro and open the prospect of analyzing pathways in vivo.

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页码：79 / 84

页数：6

相关论文

共 26 条

[1]

[Anonymous], 1985, ENZYME STRUCTURE MEC

[2] TOWARD SOLVING THE FOLDING PATHWAY OF BARNASE - THE COMPLETE BACKBONE C-13, N-15, AND H-1-NMR ASSIGNMENTS OF ITS PH-DENATURED STATE [J].

ARCUS, VL ;

VUILLEUMIER, S ;

FREUND, SMV ;

BYCROFT, M ;

FERSHT, AR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (20) :9412-9416

[3] DETECTION AND CHARACTERIZATION OF A FOLDING INTERMEDIATE IN BARNASE BY NMR [J].

BYCROFT, M ;

MATOUSCHEK, A ;

KELLIS, JT ;

SERRANO, L ;

FERSHT, AR .

NATURE, 1990, 346 (6283) :488-490

[4] MOLECULAR-DYNAMICS SIMULATION OF PROTEIN DENATURATION - SOLVATION OF THE HYDROPHOBIC CORES AND SECONDARY STRUCTURE OF BARNASE [J].

CAFLISCH, A ;

KARPLUS, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (05) :1746-1750

[5] ROLES OF MOLECULAR CHAPERONES IN PROTEIN-FOLDING [J].

ELLIS, RJ .

CURRENT OPINION IN STRUCTURAL BIOLOGY, 1994, 4 (01) :117-122

[6] PROTEIN FOLDING STUDIED USING HYDROGEN-EXCHANGE LABELING AND 2-DIMENSIONAL NMR [J].

ENGLANDER, SW ;

MAYNE, L .

ANNUAL REVIEW OF BIOPHYSICS AND BIOMOLECULAR STRUCTURE, 1992, 21 :243-265

[7] SINGLE VERSUS PARALLEL PATHWAYS OF PROTEIN-FOLDING AND FRACTIONAL FORMATION OF STRUCTURE IN THE TRANSITION-STATE [J].

FERSHT, AR ;

ITZHAKI, LS ;

ELMASRY, N ;

MATTHEWS, JM ;

OTZEN, DE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (22) :10426-10429

[8] PROTEIN-FOLDING AND STABILITY - THE PATHWAY OF FOLDING OF BARNASE [J].

FERSHT, AR .

FEBS LETTERS, 1993, 325 (1-2) :5-16

[9] THE FOLDING OF AN ENZYME .1. THEORY OF PROTEIN ENGINEERING ANALYSIS OF STABILITY AND PATHWAY OF PROTEIN FOLDING [J].

FERSHT, AR ;

MATOUSCHEK, A ;

SERRANO, L .

JOURNAL OF MOLECULAR BIOLOGY, 1992, 224 (03) :771-782

[10] PATHWAY AND STABILITY OF PROTEIN-FOLDING [J].

FERSHT, AR .

BIOCHEMICAL SOCIETY TRANSACTIONS, 1994, 22 (02) :267-273