THE TRANSPORT OF VITAMIN-B12 THROUGH POLARIZED MONOLAYERS OF CACO-2 CELLS

被引：108

作者：

DIX, CJ ^{[1
]}

HASSAN, IF ^{[1
]}

OBRAY, HY ^{[1
]}

SHAH, R ^{[1
]}

WILSON, G ^{[1
]}

机构：

[1] CIBA GEIGY PHARMACEUT CORP,ADV DRUG DELIVERY RES UNIT,WIMBLEHURST RD,HORSHAM RH12 4AB,W SUSSEX,ENGLAND

来源：

GASTROENTEROLOGY | 1990年 / 98卷 / 05期

关键词：

D O I：

10.1016/0016-5085(90)90344-Z

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Caco-2 cells grown on 0.45-μm filters, in Millicell chambers, form intact monolayers with many of the properties of polarized intestinal epithelial cells. It is reported here that these cells bind and internalize intrinsic factor-cobalamin complexes and that after 14-28 days in culture this specific binding is exclusively located on the apical membrane. Caco-2 cells also synthesize and secrete a protein with properties similar to transcobalamin II. This protein is secreted from the basolateral side of the cells after 20 days in culture. Specific apical-to-basolateral transcellular transport of [57Co]cobalamin also occurs between 20 and 28 days in culture. Thus, Caco-2 cells provide the first polarized human cell system for studying the transepithelial transport of cobalamin. © 1990.

引用

页码：1272 / 1279

页数：8

共 23 条

[1] CORRECTION OF COBALAMIN MALABSORPTION IN PANCREATIC INSUFFICIENCY WITH A COBALAMIN ANALOG THAT BINDS WITH HIGH AFFINITY TO R-PROTEIN BUT NOT TO INTRINSIC-FACTOR - INVIVO EVIDENCE THAT A FAILURE TO PARTIALLY DEGRADE R-PROTEIN IS RESPONSIBLE FOR COBALAMIN MALABSORPTION IN PANCREATIC INSUFFICIENCY [J].