THE EFFECTS OF RECOMBINANT HUMAN INTERLEUKIN-1-BETA ON CELLULAR PROLIFERATION AND THE PRODUCTION OF PROSTAGLANDIN-E2, PLASMINOGEN-ACTIVATOR, OSTEOCALCIN AND ALKALINE-PHOSPHATASE BY OSTEOBLAST-LIKE CELLS DERIVED FROM HUMAN-BONE

被引：115

作者：

EVANS, DB ^{[1
]}

BUNNING, RAD ^{[1
]}

RUSSELL, RGG ^{[1
]}

机构：

[1] UNIV SHEFFIELD,SCH MED,DEPT HUMAN METAB & CLIN BIOCHEM,SHEFFIELD S10 2RX,S YORKSHIRE,ENGLAND

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 166卷 / 01期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1016/0006-291X(90)91932-I

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

There is mounting evidence implicating cytokines such as interleukin-1 in the local regulation of bone homeostasis. In this report we show that recombinant human interleukin-1β (rhIL-1β) influences several activities of osteoblast-like cells derived from human trabecular bone explants in vitro. rhIL-1β stimulated cellular proliferation and the synthesis of prostaglandin E2 and plasminogen activator activity in the cultured human osteoblast-like cells in a dose-dependent manner. However, the induction of osteocalcin synthesis and alkaline phosphatase activity in response to 1,25(OH)2D3, two characteristics of the osteoblast phenotype, were antagonized by rhIL-1β over a similar dose range. This study adds further support to the potential role of interleukin-1 in the physiological and pathological modulation of bone cell metabolism. © 1990.

引用

页码：208 / 216

页数：9