共 31 条
[31]
WONG DTO, 1956, J AM CHEM SOC, V78, P3230
FLUOROMETRIC ASSAY OF ENZYMATIC REACTIONS INVOLVING ACETYL COENZYME A IN ALDOL CONDENSATIONS
被引：31
作者：
CALVO, JM
BARTHOLO.JC
STIEGLIT.BI
机构：
[1] Department of Biochemistry and Molecular Biology, Cornell University, Ithaca
基金：
美国国家科学基金会;
美国国家卫生研究院;
关键词：
D O I：
10.1016/0003-2697(69)90168-7
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ;
081704 ;
摘要：
The synthesis and properties (NMR, UV absorption, fluorescence emission spectra, and fluorescence quantum yield) of umbelliferone, 4-isopropylumbelliferone, and 4-carboxymethylumbelliferone are described. The optimal conditions for the formation of these umbelliferones from substituted malic acids and resorcinol have been determined. This forms the basis for a rapid and sensitive fluoremetric assay of enzymes catalyzing aldol condensation reactions involving acetyl coenzyme A. © 1969.
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页码：164 / &
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