CLONAL DELETION OF THYMIC MATURE T-CELLS INDUCED BY STAPHYLOCOCCAL ENTEROTOXIN-B IN MURINE FETAL THYMUS ORGAN-CULTURE

被引:8
作者
AIBA, Y
MAZDA, O
MATSUZAKI, Y
NAKAUCHI, H
MURAMATSU, S
KATSURA, Y
机构
[1] KYOTO UNIV,FAC SCI,CHEST DIS RES INST,DEPT IMMUNOL,KYOTO 606,JAPAN
[2] KYOTO UNIV,FAC SCI,DEPT ZOOL,KYOTO 606,JAPAN
[3] INST PHYS & CHEM RES,WAKO,SAITAMA 351,JAPAN
关键词
T-CELL TOLERANCE; FETAL THYMUS ORGAN CULTURE; STAPHYLOCOCCAL ENTEROTOXIN-B; CLONAL DELETION; SUPERANTIGEN;
D O I
10.1002/eji.1830230407
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The present study aims at investigating the intrathymic maturational stage of T cells at which clonal deletion can be induced. Staphylococcal enterotoxin B (SEB) was added to organ cultures of murine fetal thymus lobes at various time points of culture, and Vbeta8-expressing cells were assayed on day 14. Vbeta8 low-expressing (Vbeta8lo) cells were reduced to 40-60 % of the control receiving no SEB, though the reduction was ambiguous when SEB was given on day 13. In marked contrast, Vbeta8 high-expressing (Vbeta8hi) cells were virtually completely deleted in all groups including the group given SEB on day 13. Most of the Vbeta8hi cells that were deleted by 24 h of treatment with SEB were shown to be of the CD4+8- mature phenotype. though CD4-8+Vbeta8hi cells were also deleted. It was further shown that the thymic Vbeta8hiCD4+8- cells recovered from organs cultured for 14 days without SEB responded to immobilized anti-Vbeta8 monoclonal antibody, indicating that Vbeta8hi cells, which were highly sensitive to clonal deletion, were functionally competent mature T cells. These results strongly suggest that the thymus is capable of eliminating all T cells recognizing antigen present in the thymus regardless of the maturational stage of T cells.
引用
收藏
页码:815 / 819
页数:5
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