PARTICIPATION OF THE HUMAN BETA-GLOBIN LOCUS-CONTROL REGION IN INITIATION OF DNA-REPLICATION

被引：189

作者：

ALADJEM, MI

GROUDINE, M

BRODY, LL

DIEKEN, ES

FOURNIER, REK

WAHL, GM

EPNER, EM

机构：

[1] SALK INST, GENE EXPRESS LAB, SAN DIEGO, CA 92037 USA

[2] FRED HUTCHINSON CANC RES CTR, SEATTLE, WA 98195 USA

[3] UNIV WASHINGTON, SEATTLE, WA 98195 USA

来源：

SCIENCE | 1995年 / 270卷 / 5237期

关键词：

D O I：

10.1126/science.270.5237.815

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The human beta-globin locus control region (LCR) controls the transcription, chromatin structure, and replication timing of the entire locus. DNA replication was found to initiate in a transcription-independent manner within a region located 50 kilobases downstream of the LCR in human, mouse, and chicken cells containing the entire human beta-globin locus. However, DNA replication did not initiate within a deletion mutant locus lacking the sequences that encompass the LCR. This mutant locus replicated in the 3' to 5' direction. Thus, interactions between distantly separated sequences can be required for replication initiation, and factors mediating this interaction appear to be conserved in evolution.

引用

页码：815 / 819

页数：5

共 37 条

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ALADJEM MI, UNPUB

[2]

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