MODELING OF POLIOVIRUS - HIV-1 ANTIGEN CHIMERAS

被引：22

作者：

CRABBE, MJC

EVANS, DJ

ALMOND, JW

机构：

[1] Department of Microbiology, School of Animal and Microbiol Sciences, University of Reading, Reading, RG1 5 AQ, London Road

来源：

FEBS LETTERS | 1990年 / 271卷 / 1-2期

基金：

英国惠康基金;

关键词：

AIDS virus; Antibody reaction; Glycoprotein; Molecular modelling; Turn; Vaccine; β-Hairpin;

D O I：

10.1016/0014-5793(90)80404-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have used laboratory-based molecular modelling to identify structural features of antigen chimaeras of poliovirus expressing epitopes from human immunodeficiency virus (HIV-1) that may affect virus viability. Chimaeras were constructed by replacement of antigenic site 1 of VP1 by sequences corresponding to epitopes from HIV-1. Loop volume, estimated by approximating the loop to an ellipsoid was significantly (P < 0.001) lower in viable (2062.1 Å3 ± 400.2) than in non-viable (3617 Å3 ± 650.7) constructs. Our results suggest that viable virus will only be formed when antigen chimearas modified at antigenic site of VP1 have a loop occupying a similar volume in space to that occupied by the antigenic site 1 loop. In addition, the modified loop must fit with the peptide bond angles and distances at the top of the β-barrel of VP1. © 1990.

引用

页码：194 / 198

页数：5

共 19 条

[11] DELINEATION OF A REGION OF THE HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 GP120 GLYCOPROTEIN CRITICAL FOR INTERACTION WITH THE CD4 RECEPTOR [J].

LASKY, LA ;

NAKAMURA, G ;

SMITH, DH ;

FENNIE, C ;

SHIMASAKI, C ;

PATZER, E ;

BERMAN, P ;

GREGORY, T ;

CAPON, DJ .

CELL, 1987, 50 (06) :975-985

[12] MODELING ANTIBODY HYPERVARIABLE LOOPS - A COMBINED ALGORITHM [J].

MARTIN, ACR ;

CHEETHAM, JC ;

REES, AR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (23) :9268-9272

[13] ANTIGENIC STRUCTURE OF POLIOVIRUSES OF SEROTYPE-1, SEROTYPE-2 AND SEROTYPE-3 [J].

MINOR, PD ;

FERGUSON, M ;

EVANS, DMA ;

ALMOND, JW ;

ICENOGLE, JP .

JOURNAL OF GENERAL VIROLOGY, 1986, 67 :1283-1291

[14]

MYERS G, 1989, HUMAN RETROVIRUSES A

[15] STRUCTURE OF A HUMAN COMMON COLD VIRUS AND FUNCTIONAL-RELATIONSHIP TO OTHER PICORNAVIRUSES [J].

ROSSMANN, MG ;

ARNOLD, E ;

ERICKSON, JW ;

FRANKENBERGER, EA ;

GRIFFITH, JP ;

HECHT, HJ ;

JOHNSON, JE ;

KAMER, G ;

LUO, M ;

MOSSER, AG ;

RUECKERT, RR ;

SHERRY, B ;

VRIEND, G .

NATURE, 1985, 317 (6033) :145-153

[16] DEFINITION OF GENERAL TOPOLOGICAL EQUIVALENCE IN PROTEIN STRUCTURES - A PROCEDURE INVOLVING COMPARISON OF PROPERTIES AND RELATIONSHIPS THROUGH SIMULATED ANNEALING AND DYNAMIC-PROGRAMMING [J].

SALI, A ;

BLUNDELL, TL .

JOURNAL OF MOLECULAR BIOLOGY, 1990, 212 (02) :403-428

[17] CONFORMATION OF BETA-HAIRPINS IN PROTEIN STRUCTURES - A SYSTEMATIC CLASSIFICATION WITH APPLICATIONS TO MODELING BY HOMOLOGY, ELECTRON-DENSITY FITTING AND PROTEIN ENGINEERING [J].

SIBANDA, BL ;

BLUNDELL, TL ;

THORNTON, JM .

JOURNAL OF MOLECULAR BIOLOGY, 1989, 206 (04) :759-777

[18] LOCATION OF CONTINUOUS ANTIGENIC DETERMINANTS IN THE PROTRUDING REGIONS OF PROTEINS [J].

THORNTON, JM ;

EDWARDS, MS ;

TAYLOR, WR ;

BARLOW, DJ .

EMBO JOURNAL, 1986, 5 (02) :409-413

[19]

Vinter J G, 1987, J Comput Aided Mol Des, V1, P31, DOI 10.1007/BF01680556

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