A NOVEL HUMAN INSULIN-LIKE GROWTH-FACTOR BINDING-PROTEIN SECRETED BY OSTEOBLAST-LIKE CELLS

被引：81

作者：

ANDRESS, DL

BIRNBAUM, RS

机构：

[1] VET AFFAIRS MED CTR,CTR GERIATR RES EDUC & CLIN,TACOMA,WA

[2] VET AFFAIRS MED CTR,RES SERV,TACOMA,WA 98493

[3] UNIV WASHINGTON,DEPT MED,SEATTLE,WA 98195

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 176卷 / 01期

关键词：

D O I：

10.1016/0006-291X(91)90911-P

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Insulin-like growth factor binding proteins (IGFBPs) modulate the cellular action of the insulin-like growth factors. Inhibition or enhancement of IGF effects by these cell-secreted binding proteins have been described. We have purified two IGFBPs (23 and 29 kDa) from media conditioned by U-2 human osteosarcoma cells using ligand-affinity chromatography and reversed phase HPLC. N-terminal amino acid analysis of the 23 kDa protein revealed a unique sequence with variable homology to IGFBPs 1-4. The 29 kDa IGFBP was found to be nearly identical to a recently reported IGFBP. Because the affinity purified U-2 IGFBPs enhanced IGF-I-stimulated osteoblast mitogenesis, we suggest that one or both of these binding proteins enhance IGF action in bone. © 1991.

引用

收藏

页码：213 / 218

页数：6

相关论文

共 26 条

[11] INSULIN-LIKE GROWTH FACTOR-I HAS INDEPENDENT EFFECTS ON BONE-MATRIX FORMATION AND CELL REPLICATION [J].

HOCK, JM ;

CENTRELLA, M ;

CANALIS, E .

ENDOCRINOLOGY, 1988, 122 (01) :254-260

[12] ANALYSIS OF SERUM INSULIN-LIKE GROWTH-FACTOR BINDING-PROTEINS USING WESTERN BLOTTING - USE OF THE METHOD FOR TITRATION OF THE BINDING-PROTEINS AND COMPETITIVE-BINDING STUDIES [J].

HOSSENLOPP, P ;

SEURIN, D ;

SEGOVIAQUINSON, B ;

HARDOUIN, S ;

BINOUX, M .

ANALYTICAL BIOCHEMISTRY, 1986, 154 (01) :138-143

[13] INHIBITION OF BIOLOGICAL-ACTIVITY OF MULTIPLICATION-STIMULATING ACTIVITY BY BINDING TO ITS CARRIER PROTEIN [J].

KNAUER, DJ ;

SMITH, GL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (12) :7252-7256

[14] INHIBITORY INSULIN-LIKE GROWTH FACTOR-BINDING PROTEIN - CLONING, COMPLETE SEQUENCE, AND PHYSIOLOGICAL REGULATION [J].

LATOUR, D ;

MOHAN, S ;

LINKHART, TA ;

BAYLINK, DJ ;

STRONG, DD .

MOLECULAR ENDOCRINOLOGY, 1990, 4 (12) :1806-1814

[15]

MARTIN JL, 1990, J BIOL CHEM, V265, P4124

[16]

MATSUDAIRA P, 1987, J BIOL CHEM, V262, P10035

[17] DEVELOPMENT OF VALID METHODS TO MEASURE INSULIN-LIKE GROWTH FACTOR-I AND FACTOR-II IN BONE CELL-CONDITIONED MEDIUM [J].

MOHAN, S ;

BAUTISTA, CM ;

HERRING, SJ ;

LINKHART, TA ;

BAYLINK, DJ .

ENDOCRINOLOGY, 1990, 126 (05) :2534-2542

[18] ISOLATION OF AN INHIBITORY INSULIN-LIKE GROWTH-FACTOR (IGF) BINDING-PROTEIN FROM BONE CELL-CONDITIONED MEDIUM - A POTENTIAL LOCAL REGULATOR OF IGF ACTION [J].

MOHAN, S ;

BAUTISTA, CM ;

WERGEDAL, J ;

BAYLINK, DJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (21) :8338-8342

[19] ISOLATION FROM HUMAN CEREBROSPINAL-FLUID OF A NEW INSULIN-LIKE GROWTH FACTOR-BINDING PROTEIN WITH A SELECTIVE AFFINITY FOR IGF-II [J].

ROGHANI, M ;

HOSSENLOPP, P ;

LEPAGE, P ;

BALLAND, A ;

BINOUX, M .

FEBS LETTERS, 1989, 255 (02) :253-258

[20] INSULIN-LIKE GROWTH-FACTOR (IGF)-BINDING PROTEINS INHIBIT THE BIOLOGICAL-ACTIVITIES OF IGF-1 AND IGF-2 BUT NOT DES-(1-3)-IGF-1 [J].

ROSS, M ;

FRANCIS, GL ;

SZABO, L ;

WALLACE, JC ;

BALLARD, FJ .

BIOCHEMICAL JOURNAL, 1989, 258 (01) :267-272