A NOVEL HUMAN INSULIN-LIKE GROWTH-FACTOR BINDING-PROTEIN SECRETED BY OSTEOBLAST-LIKE CELLS

被引：81

作者：

ANDRESS, DL

BIRNBAUM, RS

机构：

[1] VET AFFAIRS MED CTR,CTR GERIATR RES EDUC & CLIN,TACOMA,WA

[2] VET AFFAIRS MED CTR,RES SERV,TACOMA,WA 98493

[3] UNIV WASHINGTON,DEPT MED,SEATTLE,WA 98195

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 176卷 / 01期

关键词：

D O I：

10.1016/0006-291X(91)90911-P

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Insulin-like growth factor binding proteins (IGFBPs) modulate the cellular action of the insulin-like growth factors. Inhibition or enhancement of IGF effects by these cell-secreted binding proteins have been described. We have purified two IGFBPs (23 and 29 kDa) from media conditioned by U-2 human osteosarcoma cells using ligand-affinity chromatography and reversed phase HPLC. N-terminal amino acid analysis of the 23 kDa protein revealed a unique sequence with variable homology to IGFBPs 1-4. The 29 kDa IGFBP was found to be nearly identical to a recently reported IGFBP. Because the affinity purified U-2 IGFBPs enhanced IGF-I-stimulated osteoblast mitogenesis, we suggest that one or both of these binding proteins enhance IGF action in bone. © 1991.

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页码：213 / 218

页数：6

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