INHIBITION OF NEUTROPHIL SUPEROXIDE PRODUCTION BY HUMAN PLASMA ALPHA-1-ANTITRYPSIN

被引：89

作者：

BUCURENCI, N ^{[1
]}

BLAKE, DR ^{[1
]}

CHIDWICK, K ^{[1
]}

WINYARD, PG ^{[1
]}

机构：

[1] UNIV LONDON LONDON HOSP,COLL MED,INFLAMMAT RES GRP,ARTHRITIS & RHEUMATISM COUNCIL BLDG,LONDON E1 2AD,ENGLAND

来源：

FEBS LETTERS | 1992年 / 300卷 / 01期

关键词：

ALPHA-ANTITRYPSIN; SERINE PROTEASE INHIBITOR; NEUTROPHIL RESPIRATORY BURST; SUPEROXIDE ANION RADICAL; INFLAMMATION; HUMAN;

D O I：

10.1016/0014-5793(92)80156-B

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report here that human plasma alpha-1-antitrypsin (alpha-1-AT) inhibited human neutrophil O2.- release elicited by a variety of stimulants. In comparison, the inhibitory capacities of two serine protease inhibitors, L-1-tosylamide 2-phenylethyl chloromethyl ketone (TPCK) and soybean trypsin inhibitor (SBTI), and the human recombinant alpha-1-AT mutant, alpha-1-AT-Arg358 were in the order: alpha-1-AT almost-equal-to TPCK much greater than alpha-1-AT-Arg358 > SBTI when cells were stimulated with concanavalin A plus cytochalasin E. These data suggest that, in human inflammatory fluids containing relatively high concentrations of alpha-1-AT (such as rheumatoid arthritis synovial fluid), (i) alpha-1-AT may down-regulate the inflammatory process by inhibiting the neutrophil respiratory burst and (ii) serpin oxidation by neutrophil-released reactive oxygen species is unlikely to occur.

引用

页码：21 / 24

页数：4

共 27 条

[1] SYNTHESIS AND ANALYTICAL USE OF A HIGHLY SENSITIVE AND CONVENIENT SUBSTRATE OF ELASTASE [J].