EARLY ENDONUCLEASE ACTIVATION FOLLOWING REVERSIBLE FOCAL ISCHEMIA IN THE RAT-BRAIN

被引：81

作者：

CHARRIAUTMARLANGUE, C ^{[1
]}

MARGAILL, I ^{[1
]}

PLOTKINE, M ^{[1
]}

BENARI, Y ^{[1
]}

机构：

[1] UNIV PARIS 05,PHARMACOL LAB,PARIS,FRANCE

来源：

JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM | 1995年 / 15卷 / 03期

关键词：

APOPTOSIS; CALCIUM; NECROSIS; PULSED-FIELD ELECTROPHORESIS;

D O I：

10.1038/jcbfm.1995.48

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The structural changes that occur in chromatin DNA after ischemic brain injury are poorly understood. The presence of oligonucleosome fragments that are recognized as the characteristic DNA ladder has been demonstrated in global and focal ischemia, associated or not with random DNA fragmentation. Using pulsed-field gel electrophoresis, which improves DNA separation, we have now detected initial stages of DNA fragmentation that occur already 6 h after reversible focal cerebral ischemia in rats. This result confirms that internucleosomal DNA fragmentation precedes random DNA fragmentation in vulnerable striatal and cortical neurons following reversible focal cerebral ischemia.

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页码：385 / 388

页数：4

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