DNA-DAMAGE CONSISTENT WITH APOPTOSIS IN TRANSIENT FOCAL ISCHEMIC NEOCORTEX

被引：184

作者：

MACMANUS, JP ^{[1
]}

HILL, IE ^{[1
]}

HUANG, ZG ^{[1
]}

RASQUINHA, I ^{[1
]}

XUE, D ^{[1
]}

BUCHAN, AM ^{[1
]}

机构：

[1] CIV HOSP,LOEB INST,OTTAWA K1Y 4E9,ON,CANADA

来源：

NEUROREPORT | 1994年 / 5卷 / 04期

关键词：

APOPTOSIS; BRAIN; CEREBRAL CORTEX; DNA DAMAGE; ISCHEMIA; TERMINAL DEOXYRIBONUCLEOTIDYLTRANSFERASE;

D O I：

10.1097/00001756-199401120-00031

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

TRANSIENT focal ischaemia was produced in rat right neocortex by temporary middle cerebral artery occlusion. DNA damage was visualized in situ in cells of this right hemisphere but not in the contralateral hemisphere. The extracted damaged DNA exhibited laddered fragmentation which is indicative of apoptotic degradation. The amount of DNA damage was quantified by an end-labelling technique and shown to increase with the duration of the ischaemic insult. We conclude that the neurodegeneration resulting from focal ischaemia has an apoptotic component.

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页码：493 / 496

页数：4

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