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ISOLATION OF A HUMAN YAC CONTIG ENCOMPASSING A CLUSTER OF UGT2 GENES AND ITS REGIONAL LOCALIZATION TO CHROMOSOME 4Q13

被引：53
作者：
MONAGHAN, G
CLARKE, DJ
POVEY, S
SEE, CG
BOXER, M
BURCHELL, B
机构：
[1] UNIV DUNDEE, NINEWELLS HOSP & MED SCH, DEPT BIOCHEM MED, DUNDEE DD1 9SY, SCOTLAND
[2] UNIV DUNDEE, NINEWELLS HOSP & MED SCH, DEPT PATHOL, DUNDEE DD1 9SY, SCOTLAND
[3] UNIV LONDON UNIV COLL, DEPT GENET & BIOMETRY, GALTON LAB, LONDON NW1 2HE, ENGLAND
[4] UNIV LONDON UNIV COLL, MRC, HUMAN BIOCHEM GENET UNIT, LONDON NW1 2HE, ENGLAND
来源：
GENOMICS | 1994年 / 23卷 / 02期
基金：
英国惠康基金;
关键词：
D O I：
10.1006/geno.1994.1531
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Previously we mapped the gene encoding a human bile acid UDP-glucuronosyltransferase (UGT2B4) to chromosome 4. Here we report the mapping of two additional human UGT2B genes to chromosome 4 utilizing the polymerase chain reaction (PCR) and a panel of human/rodent somatic cell hybrid cell lines. A yeast artificial chromosome contig containing the UGT2B4, UGT2B9, and UGT2B15 genes was isolated, and pulsed-field gel electrophoresis and PCR revealed that several members of the human UGT2B gene subfamily are clustered within a 195-kb region of the YAC contig. These data permitted a provisional ordering of the genes as UGT2B9-UGT2B4-UGT2B15. Fluorescence in situ hybridization analysis, using the YAC DNA, permitted the regional localization of this gene cluster to chromosome 4q13. (C) 1994 Academic Press, Inc.
引用
收藏
页码：496 / 499
页数：4
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