FUNCTIONAL-CHARACTERIZATION OF ALPHA-ADRENOCEPTORS ON PANCREATIC-ISLETS OF FA/FA ZUCKER RATS

被引：5

作者：

CHAN, CB

MACPHAIL, RM

机构：

[1] Department of Anatomy and Physiology, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown

来源：

MOLECULAR AND CELLULAR ENDOCRINOLOGY | 1992年 / 84卷 / 1-2期

基金：

英国医学研究理事会;

关键词：

OBESITY; ALPHA-ADRENERGIC RECEPTOR; CLONIDINE; PRAZOSIN; YOHIMBINE;

D O I：

10.1016/0303-7207(92)90068-H

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Recently, a defect in pertussis toxin-independent actions of epinephrine on pancreatic B-cells of fa/fa Zucker rats was reported (Cawthorn and Chan (1991) Mol. Cell. Endocrinol. 75, 197-204). We now report studies of islet alpha(2)-adrenoceptor function of fa/fa rats. Insulin and cAMP production by islets of obese rats were both inhibited by the alpha(2)-adrenoceptor agonist clonidine. Calculated pD2 values for clonidine were 9.57 +/- 0.59 and 9.43 +/- 0.33 for lean and fa/fa rat islets, respectively. Yohimbine reversed clonidine effects equipotently in lean and obese rat islets (pA2 values of 7.48 +/- 0.57 vs 7.43 +/- 0.58). Unexpectedly, the alpha(1)-antagonist prazosin stimulated insulin secretion from islets of obese but not lean rats. Functional characteristics of the alpha-adrenoceptors on fa/fa islets are thus similar to those recently designated alpha(2B). Altered expression of alpha-adrenoceptors on pancreatic islets of fa/fa rats may contribute to changes in the pertussis toxin-independent pathway of epinephrine action previously observed.

引用

页码：33 / 37

页数：5

共 15 条

[11]

NIDDAM R, 1990, J PHARMACOL EXP THER, V254, P883

[12] ALPHA-ADRENOCEPTORS AND INSULIN RELEASE FROM PANCREATIC-ISLETS OF NORMAL AND DIABETIC RATS [J].

OSTENSON, CG ;

CATTANEO, AG ;

DOXEY, JC ;

EFENDIC, S .

AMERICAN JOURNAL OF PHYSIOLOGY, 1989, 257 (03) :E439-E443

[13]

RUFFOLO RR, 1987, ALPHA 2 ADRENERGIC R, P115

[14] NON CLASSICAL, MULTIPLE-SITE INTERACTION OF [H-3] PRAZOSIN WITH THE ALPHA-1-ADRENOCEPTOR OF INTACT BC3H1 CELLS [J].

SLADECZEK, F ;

KIRK, CJ ;

BOCKAERT, J ;

SCHMIDT, BH .

BRITISH JOURNAL OF PHARMACOLOGY, 1989, 97 (04) :1101-1110

[15]

VANDERVLIET JA, 1988, TRANSPLANTATION, V45, P493

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