ISOLATION OF TSH AND LH CG RECEPTOR CDNAS FROM HUMAN THYROID - REGULATION BY TISSUE SPECIFIC SPLICING

被引：161

作者：

FRAZIER, AL

ROBBINS, LS

STORK, PJ

SPRENGEL, R

SEGALOFF, DL

CONE, RD

机构：

[1] OREGON HLTH SCI UNIV, VOLLUM INST ADV BIOMED RES, 3181 SW SAM JACKSON PK RD, PORTLAND, OR 97201 USA

[2] NEW ENGLAND MED CTR HOSP, DIV MOLEC MED, BOSTON, MA 02111 USA

[3] UNIV HEIDELBERG, ZENTRUM MOLEK BIOL HEIDELBERG, W-6900 HEIDELBERG, GERMANY

[4] CHILDRENS HOSP MED CTR, DIV HEMATOL ONCOL, BOSTON, MA 02115 USA

[5] POPULAT COUNCIL, NEW YORK, NY 10021 USA

[6] NEW ENGLAND MED CTR HOSP, DEPT PATHOL, BOSTON, MA 02111 USA

来源：

MOLECULAR ENDOCRINOLOGY | 1990年 / 4卷 / 08期

关键词：

D O I：

10.1210/mend-4-8-1264

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

A TSH receptor (TSH-R) cDNA has been isolated from a human thyroid λGT11 library. Unexpectedly, several cDNAs encoding the human LH/CG receptor (LH/CG-R), previously thought to be expressed solely in gonadal cells, were also isolated from the thyroid library. The receptors are structurally related, consisting of a signal sequence, a large extracellular amino terminal domain, seven membrane spanning domains, and a short carboxyl-terminal portion. The TSH-R is encoded by a single 4.2 kilobase mRNA specific to the thyroid. Introns were not present in any hTSH-R cDNAs examined, however, sequencing of several LH/CG-R cDNAs and RNase protection experiments demonstrated that the majority of hLH/ CG-R mRNA in the thyroid is incompletely spliced. Consequently, tissue-specific splicing may be an important step in the regulation of the glycoprotein hormone receptor family. © 1990 by The Endocrine Society.

引用

页码：1264 / 1276

页数：13

共 67 条

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