A GENE-EXPRESSION SCREEN

被引：305

作者：

WANG, Z

BROWN, DD

机构：

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 24期

关键词：

CDNA SUBTRACTION; AMPHIBIAN METAMORPHOSIS; TAIL RESORPTION; GENE EXPRESSION; THYROID HORMONE;

D O I：

10.1073/pnas.88.24.11505

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A gene expression screen identifies mRNAs that differ in abundance between two mRNA mixtures by a subtractive hybridization method. The two mRNA populations are converted to double-stranded cDNAs, fragmented, and ligated to linkers for polymerase chain reaction (PCR) amplification. The multiple cDNA fragments isolated from any given gene can be treated as alleles in a genetic screen. Probability analysis of the frequency with which multiple alleles are found provides an estimation of the total number of up- and down-regulated genes. We have applied this method to genes that are differentially expressed in amphibian tadpole tail tissue in the first 24 hr after thyroid hormone treatment, which ultimately induces tail resorption. We estimate that there are about 30 up-regulated genes; 16 have been isolated.

引用

页码：11505 / 11509

页数：5

共 22 条

[11]

Maniatis T., 1982, MOL CLONING

[12] EXPRESSION OF J-CHAIN RNA IN CELL-LINES REPRESENTING DIFFERENT STAGES OF LYMPHOCYTE-B DIFFERENTIATION [J].