CARBOXYL METHYLATION OF PROTEIN PHOSPHATASE 2A FROM XENOPUS EGGS IS STIMULATED BY CAMP AND INHIBITED BY OKADAIC ACID

被引：38

作者：

FLOER, M

STOCK, J

机构：

[1] Department of Molecular Biology, Princeton University, Princeton

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 198卷 / 01期

关键词：

D O I：

10.1006/bbrc.1994.1052

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Protein phosphatase 2A is the principal carboxyl methylated protein in cytoplasmic extracts derived from Xenopus eggs arrested in interphase. Levels of methylation approach stoichiometric values after 2 hr of incubation at 22°C. Rates of PP2A methylation increase by over 50% upon addition of cAMP. Methylation is inhibited by okadaic acid at concentrations that inhibit PP2A phosphatase activity. © 1994 Academic Press, Inc.

引用

收藏

页码：372 / 379

页数：8

相关论文

共 14 条

[1]

BINSTOCK J, 1984, METHOD ENZYMOL, V106, P310

[2] METHYL-ESTERIFIED PROTEINS IN A MAMMALIAN-CELL LINE [J].

CHELSKY, D ;

RUSKIN, B ;

KOSHLAND, DE .

BIOCHEMISTRY, 1985, 24 (23) :6651-6658

[3] PROTEIN ISOPRENYLATION AND METHYLATION AT CARBOXYL-TERMINAL CYSTEINE RESIDUES [J].

CLARKE, S .

ANNUAL REVIEW OF BIOCHEMISTRY, 1992, 61 :355-386

[4] THE STRUCTURE AND REGULATION OF PROTEIN PHOSPHATASES [J].

COHEN, P .

ANNUAL REVIEW OF BIOCHEMISTRY, 1989, 58 :453-508

[5] OKADAIC ACID - A NEW PROBE FOR THE STUDY OF CELLULAR-REGULATION [J].

COHEN, P ;

HOLMES, CFB ;

TSUKITANI, Y .

TRENDS IN BIOCHEMICAL SCIENCES, 1990, 15 (03) :98-102

[6] PROTEIN PHOSPHATASE-2A FROM XENOPUS OOCYTES - CHARACTERIZATION DURING MEIOTIC CELL-DIVISION [J].

CORMIER, P ;

OSBORNE, HB ;

BASSEZ, T ;

POULHE, R ;

BELLE, R ;

MULNERLORILLON, O .

FEBS LETTERS, 1991, 295 (1-3) :185-188

[7] A POST-RIBOSOMAL SUPERNATANT FROM ACTIVATED XENOPUS EGGS THAT DISPLAYS POST-TRANSLATIONALLY REGULATED OSCILLATION OF ITS CDC2+ MITOTIC KINASE-ACTIVITY [J].

FELIX, MA ;

PINES, J ;

HUNT, T ;

KARSENTI, E .

EMBO JOURNAL, 1989, 8 (10) :3059-3069

[8] CDC2 H1 KINASE IS NEGATIVELY REGULATED BY A TYPE-2A PHOSPHATASE IN THE XENOPUS EARLY EMBRYONIC-CELL CYCLE - EVIDENCE FROM THE EFFECTS OF OKADAIC ACID [J].

FELIX, MA ;

COHEN, P ;

KARSENTI, E .

EMBO JOURNAL, 1990, 9 (03) :675-683

[9]

GORDON RK, 1987, METHOD ENZYMOL, V143, P191

[10]

HAN JH, 1993, J BIOL CHEM, V268, P25009