ENDOTHELIN-1 INDUCES PROSTACYCLIN RELEASE FROM BOVINE AORTIC ENDOTHELIAL-CELLS

被引：89

作者：

FILEP, JG ^{[1
]}

BATTISTINI, B ^{[1
]}

COTE, YP ^{[1
]}

BEAUDOIN, AR ^{[1
]}

SIROIS, P ^{[1
]}

机构：

[1] UNIV SHERBROOKE,FAC MED,DEPT BIOL,SHERBROOKE J1H 5N4,QUEBEC,CANADA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 177卷 / 01期

关键词：

D O I：

10.1016/0006-291X(91)91964-E

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effects of endothelin-1 (ET-1) on the release of prostacyclin from cultured bovine aortic endothelial cells were studied. ET-1 induced a time- and dose-dependent release of 6-keto PGF1α, the stable metabolite of prostacyclin, with an apparent EC50 value of 3.0 ± 0.9 nM (n=6). ET-1 up to a concentration of 500 nM did not affect cellular integrity. Preincubation of the cells for 30 min with 10 μM indomethacin inhibited ET-1 (100 nM) - induced prostacyclin release by 90 %. These findings indicate that ET-1 can directly stimulate prostacyclin release from endothelial cells probably through a receptor mediated mechanism. © 1991.

引用

页码：171 / 176

页数：6

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