EFFICIENT MAGNESIUM-DEPENDENT HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 INTEGRASE ACTIVITY

被引：87

作者：

ENGELMAN, A ^{[1
]}

CRAIGIE, R ^{[1
]}

机构：

[1] NIDDK,MOLEC BIOL LAB,BETHESDA,MD 20892

来源：

JOURNAL OF VIROLOGY | 1995年 / 69卷 / 09期

关键词：

D O I：

10.1128/JVI.69.9.5908-5911.1995

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The integrase protein from human immunodeficiency virus type 1 (HIV-1) has generally been reported to require Mn2+ for efficient in vitro activity. We have reexamined the divalent metal ion requirements of HIV-1 integrase and find that the protein is capable of promoting efficient 3' processing and DNA strand transfer with either Mn2+ or Mg2+. The metal ion preference depended upon the reaction conditions. HIV-1 integrase displayed significantly less nonspecific nuclease activity in reaction mixtures containing Mg2+ than it did under the previously described reaction conditions with mixtures containing Mn2+.

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页码：5908 / 5911

页数：4

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