EFFICIENT MAGNESIUM-DEPENDENT HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 INTEGRASE ACTIVITY

被引：87

作者：

ENGELMAN, A ^{[1
]}

CRAIGIE, R ^{[1
]}

机构：

[1] NIDDK,MOLEC BIOL LAB,BETHESDA,MD 20892

来源：

JOURNAL OF VIROLOGY | 1995年 / 69卷 / 09期

关键词：

D O I：

10.1128/JVI.69.9.5908-5911.1995

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The integrase protein from human immunodeficiency virus type 1 (HIV-1) has generally been reported to require Mn2+ for efficient in vitro activity. We have reexamined the divalent metal ion requirements of HIV-1 integrase and find that the protein is capable of promoting efficient 3' processing and DNA strand transfer with either Mn2+ or Mg2+. The metal ion preference depended upon the reaction conditions. HIV-1 integrase displayed significantly less nonspecific nuclease activity in reaction mixtures containing Mg2+ than it did under the previously described reaction conditions with mixtures containing Mn2+.

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页码：5908 / 5911

页数：4

相关论文

共 27 条

[1] HIV-1 INTEGRASE - HIGH-LEVEL PRODUCTION AND SCREENING ASSAY FOR THE ENDONUCLEOLYTIC ACTIVITY [J].

BILLICH, A ;

SCHAUER, M ;

FRANK, S ;

ROSENWIRTH, B ;

BILLICH, S .

ANTIVIRAL CHEMISTRY & CHEMOTHERAPY, 1992, 3 (02) :113-119

[2] HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 INTEGRASE - EFFECT ON VIRAL REPLICATION OF MUTATIONS AT HIGHLY CONSERVED RESIDUES [J].

CANNON, PM ;

WILSON, W ;

BYLES, E ;

KINGSMAN, SM ;

KINGSMAN, AJ .

JOURNAL OF VIROLOGY, 1994, 68 (08) :4768-4775

[3] THE IN PROTEIN OF MOLONEY MURINE LEUKEMIA-VIRUS PROCESSES THE VIRAL-DNA ENDS AND ACCOMPLISHES THEIR INTEGRATION INVITRO [J].

CRAIGIE, R ;

FUJIWARA, T ;

BUSHMAN, F .

CELL, 1990, 62 (04) :829-837

[4]

Craigie R., 1995, HIV, V2, P53

[5] IDENTIFICATION OF AMINO-ACID-RESIDUES CRITICAL FOR ENDONUCLEASE AND INTEGRATION ACTIVITIES OF HIV-1 IN PROTEIN INVITRO [J].

DRELICH, M ;

WILHELM, R ;

MOUS, J .

VIROLOGY, 1992, 188 (02) :459-468

[6] HUMAN-IMMUNODEFICIENCY-VIRUS INTEGRATION IN A CELL-FREE SYSTEM [J].

ELLISON, V ;

ABRAMS, H ;

ROE, TY ;

LIFSON, J ;

BROWN, P .

JOURNAL OF VIROLOGY, 1990, 64 (06) :2711-2715

[7] MULTIPLE EFFECTS OF MUTATIONS IN HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 INTEGRASE ON VIRAL REPLICATION [J].

ENGELMAN, A ;

ENGLUND, G ;

ORENSTEIN, JM ;

MARTIN, MA ;

CRAIGIE, R .

JOURNAL OF VIROLOGY, 1995, 69 (05) :2729-2736

[8] IDENTIFICATION OF CONSERVED AMINO-ACID-RESIDUES CRITICAL FOR HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 INTEGRASE FUNCTION-INVITRO [J].

ENGELMAN, A ;

CRAIGIE, R .

JOURNAL OF VIROLOGY, 1992, 66 (11) :6361-6369

[9]

ENGELMAN A, UNPUB

[10] INTEGRATION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 DNA INVITRO [J].

FARNET, CM ;

HASELTINE, WA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (11) :4164-4168