INVIVO AND INVITRO EVIDENCE CONCERNING THE ROLE OF LIPID-PEROXIDATION IN THE MECHANISM OF HEPATOCYTE DEATH DUE TO CARBON-TETRACHLORIDE

被引：58

作者：

BIASI, F

ALBANO, E

CHIARPOTTO, E

CORONGIU, FP

PRONZATO, MA

MARINARI, UM

PAROLA, M

DIANZANI, MU

POLI, G

机构：

[1] UNIV TURIN,DEPT EXPTL MED & ONCOL,C SO RAFFAELLO 30,I-10125 TURIN,ITALY

[2] UNIV CAGLIARI,DEPT EXPTL BIOL,I-09100 CAGLIARI,ITALY

[3] UNIV GENOA,INST GEN PATHOL,I-16132 GENOA,ITALY

来源：

CELL BIOCHEMISTRY AND FUNCTION | 1991年 / 9卷 / 02期

关键词：

LIPID PEROXIDATION; CARBON TETRACHLORIDE; HEPATOCYTE DEATH; INTRACELLULAR CALCIUM;

D O I：

10.1002/cbf.290090208

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Isolated rat hepatocytes exposed to CCl4 showed a stimulated formation of malonaldehyde after only 30-60 min incubation. Conversely, the onset of hepatocyte death was a relatively late event, being significant only after 2-3 h of treatment. A cause-effect relationship between the two phenomena has been demonstrated by using hepatocytes isolated from rats pretreated with alpha-tocopherol. Comparable results were obtained in vivo where supplementation with alpha-tocopherol 15 h before CCl4 dosing induced a partial or complete protection against the drug's necrogenic effect, depending on the concentration of the haloalkane used. Moreover, the vitamin supplementation prevented the CCl4-induced increase of liver total calcium content, probably by blocking alterations in the liver cell plasma membranes due to lipid peroxidation.

引用

页码：111 / 118

页数：8

共 33 条

[21] STUDIES ON FATTY LIVER WITH ISOLATED HEPATOCYTES .2. ACTION OF CARBON-TETRACHLORIDE ON LIPID PEROXIDATION, PROTEIN, AND TRIGLYCERIDE SYNTHESIS AND SECRETION [J].