THE E2A GENE-PRODUCT CONTAINS 2 SEPARABLE AND FUNCTIONALLY DISTINCT TRANSCRIPTION ACTIVATION DOMAINS

被引：104

作者：

ARONHEIM, A ^{[1
]}

SHIRAN, R ^{[1
]}

ROSEN, A ^{[1
]}

WALKER, MD ^{[1
]}

机构：

[1] WEIZMANN INST SCI,DEPT BIOCHEM,IL-76100 REHOVOT,ISRAEL

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1993年 / 90卷 / 17期

关键词：

PANCREATIC BETA-CELLS; HELIX-LOOP-HELIX; INSULIN;

D O I：

10.1073/pnas.90.17.8063

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The E2A gene encodes transcription factors of the helix-loop-helix (HLH) family which are implicated in cell-specific transcriptional control in several cell lineages, including pancreatic beta cells. In the present work, we show by deletion mapping of both the E2A protein itself and the Gal4-E2A fusion protein that the protein contains at least two distinct activation domains. One domain (located between amino acids 1 and 153) functions efficiently in a variety of mammalian cell lines. The second domain (located between amino acids 369 and 485) functions preferentially in pancreatic beta cell fines. The latter domain shows a pattern of heptad repeats of leucine residues characteristic of ''leucine zipper'' transcription factors; site-directed mutagenesis of leucines within this repeat led to substantial reductions in activity. The selective properties of this activation domain may contribute to cell-specific transcription directed by the E2A gene.

引用

页码：8063 / 8067

页数：5

共 57 条

[1] CELL-SPECIFIC EXPRESSION OF HELIX-LOOP-HELIX TRANSCRIPTION FACTORS ENCODED BY THE E2A GENE [J].