FUNCTIONAL ANTAGONISM BETWEEN C-JUN AND MYOD PROTEINS - A DIRECT PHYSICAL ASSOCIATION

被引：410

作者：

BENGAL, E ^{[1
]}

RANSONE, L ^{[1
]}

SCHARFMANN, R ^{[1
]}

DWARKI, VJ ^{[1
]}

TAPSCOTT, SJ ^{[1
]}

WEINTRAUB, H ^{[1
]}

VERMA, IM ^{[1
]}

机构：

[1] FRED HUTCHINSON CANC RES CTR,SEATTLE,WA 98104

来源：

CELL | 1992年 / 68卷 / 03期

关键词：

D O I：

10.1016/0092-8674(92)90187-H

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The product of the proto-oncogene Jun inhibits myogenesis. Constitutive expression of Jun in myoblasts interferes with the expression and the function of MyoD protein. In transient transfection assays Jun inhibits transactivation of the MyoD promoter, the muscle creatine kinase enhancer, and a reporter gene linked to MyoD DNA-binding sites. Conversely, MyoD suppresses the transactivation by Jun of genes linked to an AP-1 site. We demonstrate that both in vivo and in vitro MyoD and Jun proteins physically interact. Mutational analysis suggests that this interaction occurs via the leucine zipper domain of Jun and the helix-loop-helix region of MyoD.

引用

收藏

页码：507 / 519

页数：13

相关论文

共 44 条

[1] PHORBOL ESTER INDUCIBLE GENES CONTAIN A COMMON CIS ELEMENT RECOGNIZED BY A TPA-MODULATED TRANS-ACTING FACTOR [J].

ANGEL, P ;

IMAGAWA, M ;

CHIU, R ;

STEIN, B ;

IMBRA, RJ ;

RAHMSDORF, HJ ;

JONAT, C ;

HERRLICH, P ;

KARIN, M .

CELL, 1987, 49 (06) :729-739

[2]

BENBROOK DM, 1990, ONCOGENE, V5, P295

[3] THE PROTEIN ID - A NEGATIVE REGULATOR OF HELIX-LOOP-HELIX DNA-BINDING PROTEINS [J].

BENEZRA, R ;

DAVIS, RL ;

LOCKSHON, D ;

TURNER, DL ;

WEINTRAUB, H .

CELL, 1990, 61 (01) :49-59

[4] TRANSFORMING GROWTH-FACTOR-BETA REPRESSES THE ACTIONS OF MYOGENIN THROUGH A MECHANISM INDEPENDENT OF DNA-BINDING [J].

BRENNAN, TJ ;

EDMONDSON, DG ;

LI, L ;

OLSON, EN .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (09) :3822-3826

[5] GENOMIC SEQUENCING [J].

CHURCH, GM ;

GILBERT, W .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (07) :1991-1995

[6] MYOD INDUCES GROWTH ARREST INDEPENDENT OF DIFFERENTIATION IN NORMAL AND TRANSFORMED-CELLS [J].

CRESCENZI, M ;

FLEMING, TP ;

LASSAR, AB ;

WEINTRAUB, H ;

AARONSON, SA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (21) :8442-8446

[7] EXPRESSION OF A SINGLE TRANSFECTED CDNA CONVERTS FIBROBLASTS TO MYOBLASTS [J].

DAVIS, RL ;

WEINTRAUB, H ;

LASSAR, AB .

CELL, 1987, 51 (06) :987-1000

[8] THE MYOD DNA-BINDING DOMAIN CONTAINS A RECOGNITION CODE FOR MUSCLE-SPECIFIC GENE ACTIVATION [J].

DAVIS, RL ;

CHENG, PF ;

LASSAR, AB ;

WEINTRAUB, H .

CELL, 1990, 60 (05) :733-746

[9] TRANSCRIPTION FACTOR INTERACTIONS - SELECTORS OF POSITIVE OR NEGATIVE REGULATION FROM A SINGLE DNA ELEMENT [J].

DIAMOND, MI ;

MINER, JN ;

YOSHINAGA, SK ;

YAMAMOTO, KR .

SCIENCE, 1990, 249 (4974) :1266-1272

[10] DISTINCTIVE EFFECTS OF THE VIRAL ONCOGENES MYC, ERB, FPS, AND SRC ON THE DIFFERENTIATION PROGRAM OF QUAIL MYOGENIC CELLS [J].

FALCONE, G ;

TATO, F ;

ALEMA, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (02) :426-430

← 1 2 3 4 5 →